Testing Data
DNA enzyme
DNA dA-Tailing Kit
Applications
DNA sample preparationdA-Tailing of 1-5 ug of blunt ended DNA
Synonyms
DNA; DNA dA-Tailing Kit; DNA enzyme
Applicable Applications for DNA enzyme
DNA sample preparation
dA-Tailing of 1-5 ug of blunt ended DNA
dA-Tailing of 1-5 ug of blunt ended DNA
Storage Buffer
25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of Klenow Fragment (3'†'5' Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37 degree C using poly(dA-dT) :poly(dA-dT) as a template:primer.
Preparation and Storage
Store all components at -20 degree C in a non-frost-free freezer.
All components are stable for 1 year from the date of shipping when stored and handled properly.
All components are stable for 1 year from the date of shipping when stored and handled properly.
Testing Data
Testing Data
Related Product Information for DNA enzyme
The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3' end of a blunt-ended DNA fragment. This incorporated 3'-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3'-dT overhangs. The Klenow Fragment (3'-->5' Exo-) in this kit adds the 3'-dA via its DNA polymerase activity. This enzyme lacks 5'-->'3' exonuclease activity and has mutations that effectively abolish the inherent 3'†'5' exonuclease activity, thus preventing degradation of this 3'-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3'-->5' Exo-) enzyme is also available as a stand-alone enzyme.
Similar Products
Product Notes
The DNA (Catalog #AAA4156422) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA can be used in a range of immunoassay formats including, but not limited to, DNA sample preparation dA-Tailing of 1-5 ug of blunt ended DNA. Researchers should empirically determine the suitability of the DNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
