LOXL2 elisa pair set

LOXL2 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118391

• Synonyms: LOXL2; LOXL2 Matched ELISA Antibody Pair Set; Human; Human LOXL2 ELISA Pair Set; lysyl oxidase-like 2; LOR2 Matched ELISA Antibody Pair Set; Lysyl oxidase-like 2 Matched ELISA Antibody Pair Set; WS9-14 Matched ELISA Antibody Pair Set; LOXL2 elisa pair set

• Specificity: Quantitative determination of Human LOXL2

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 78.13pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of mouse anti-Human LOXL2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human LOXL2 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 270 ng of recombinant Human LOXL2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for LOXL2 elisa pair set

Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein that belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 (LOXL1, LOXL2, LOXL3, LOXL4). All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.

This LOXL2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LOXL2. It contains Human LOXL2 capture antibody, Human LOXL2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human LOXL2 protein.

References

Peng, L. et al., 2009, Carcinogenesis. 30 (10):1660-9. Hollosi, P. et al., 2009, Int J Cancer. 125 (2):318-27. Ruckert, F. et al., 2010, Int J Colorectal Dis. 25 (3):303-11. Iftikhar, M. et al., 2011, J Biol Chem. 286 (2):909-18.