LOXL2 recombinant protein

LOXL2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8118874

• Gene Names: Loxl2; 1110004B06Rik; 4930526G11Rik; 9430067E15Rik
• Purity: >80 % as determined by SDS-PAGE
• Synonyms: LOXL2; LOXL2 Protein; Mouse; Recombinant (His Tag); Mouse LOXL2/Lysyl oxidase homolog 2 Protein (His Tag); lysyl oxidase-like 2; 1110004B06Rik Protein; 4930526G11Rik Protein; 9430067E15Rik Protein; LOXL2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20 mM MES, 150 mM NaCl, pH6.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 762aa
• Sequence: Met1-Gln776

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Gln26
• Protein Construction: |A DNA sequence encoding the mouse Loxl2 (NP_201582.2) (Met1-Gln776) was expressed with a polyhistidine tag at the C-terminus
• Activity: Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine.; The specific activity is >3pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for LOXL2 recombinant protein

Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein that belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 (LOXL1, LOXL2, LOXL3, LOXL4). All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.

References

Peng, L. et al., 2009, Carcinogenesis. 30 (10):1660-9. Hollosi, P. et al., 2009, Int J Cancer. 125 (2):318-27. Ruckert, F. et al., 2010, Int J Colorectal Dis. 25 (3):303-11. Iftikhar, M. et al., 2011, J Biol Chem. 286 (2):909-18.

NCBI and Uniprot Product Information

• NCBI GI #: 225579028
• NCBI GeneID: 94352
• NCBI Accession #: NP_201582.2
• NCBI GenBank Nucleotide #: NM_033325.2
• UniProt Accession #: P58022; Q8BRE6; Q8BS86; Q8C4K0; Q9JJ39
• Molecular Weight: 81,168 Da
• NCBI Official Full Name: lysyl oxidase homolog 2
• NCBI Official Synonym Full Names: lysyl oxidase-like 2
• NCBI Official Symbol: Loxl2
• NCBI Official Synonym Symbols: 1110004B06Rik; 4930526G11Rik; 9430067E15Rik
• NCBI Protein Information: lysyl oxidase homolog 2
• UniProt Protein Name: Lysyl oxidase homolog 2
• Protein Family: Lysyl oxidase
• UniProt Gene Name: Loxl2
• UniProt Entry Name: LOXL2_MOUSE

Uniprot Description

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family.

Protein type: Oxidoreductase; EC 1.4.3.13; Cell adhesion; Secreted; Secreted, signal peptide

Cellular Component: basement membrane; chromosome; extracellular region; extracellular space; membrane; nucleoplasm; nucleus; proteinaceous extracellular matrix

Molecular Function: chromatin binding; copper ion binding; metal ion binding; methylated histone residue binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity

Biological Process: chromatin modification; collagen fibril organization; endothelial cell migration; endothelial cell proliferation; epithelial to mesenchymal transition; histone modification; negative regulation of transcription, DNA-dependent; positive regulation of chondrocyte differentiation; protein amino acid deamination; protein modification process; regulation of transcription, DNA-dependent; response to copper ion; response to hypoxia; sprouting angiogenesis; transcription, DNA-dependent