Beta-2 microglobulin ELISA Pair Set | B2M elisa pair set

Beta-2 microglobulin Matched ELISA Antibody Pair Set, Rat

Cat. Num. AAA8118545

• Synonyms: Beta-2 microglobulin; Beta-2 microglobulin Matched ELISA Antibody Pair Set; Rat; Rat Beta-2 microglobulin/B2M ELISA Pair Set; beta-2 microglobulin; B2M elisa pair set

• Specificity: Quantitative determination of Rat Beta-2 microglobulin

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 2.66-170pg/mL
• Sensitivity: 2.66pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Rat Beta-2 microglobulin/B2M monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Rat Beta-2 microglobulin/B2M monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 35 ng of recombinant Rat Beta-2 microglobulin/B2M. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 170 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for B2M elisa pair set

B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (beta2-Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the alpha3 chain. Unlike alpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.

This Beta-2 microglobulin Matched ELISA Antibody Pair Set, Rat is a solid phase sandwich ELISA for quantitative determination of Rat Beta-2 microglobulin. It contains Rat Beta-2 microglobulin capture antibody, Rat Beta-2 microglobulin detector antibody (HRP) and a highly purified HEK293-expressed recombinant Rat Beta-2 microglobulin protein.

References

Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.