Rabbit anti-Human LOXL2 Polyclonal Antibody | anti-LOXL2 antibody

Anti-LOXL2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103287

• Gene Names: LOXL2; LOR2; WS9-14
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: LOXL2; Polyclonal Antibody; Anti-LOXL2 Antibody; Rabbit Polyclonal; LOXL2/Lysyl oxidase-like 2 Antibody; Rabbit PAb; Antigen Affinity Purified; lysyl oxidase-like 2; Anti-LOR2 Antibody; Anti-Lysyl oxidase-like 2 Antibody; Anti-WS9-14 Antibody; anti-LOXL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LOXL2/Lysyl oxidase-like 2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LOXL2 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human LOXL2/Lysyl oxidase-like 2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human LOXL2/Lysyl oxidase-like 2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human LOXL2 in human prostate with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-LOXL2 antibody

Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein that belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 (LOXL1, LOXL2, LOXL3, LOXL4). All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.

References

Peng, L. et al., 2009, Carcinogenesis. 30 (10):1660-9. Hollosi, P. et al., 2009, Int J Cancer. 125 (2):318-27. Ruckert, F. et al., 2010, Int J Colorectal Dis. 25 (3):303-11. Iftikhar, M. et al., 2011, J Biol Chem. 286 (2):909-18.

NCBI and Uniprot Product Information

• NCBI GI #: 4505011
• NCBI GeneID: 4017
• NCBI Accession #: NP_002309.1
• NCBI GenBank Nucleotide #: NM_002318.2
• UniProt Accession #: Q9Y4K0; Q53HV3; Q9BW70; Q9Y5Y8; B2R5Q0
• Molecular Weight: 86,725 Da
• NCBI Official Full Name: lysyl oxidase homolog 2
• NCBI Official Synonym Full Names: lysyl oxidase like 2
• NCBI Official Symbol: LOXL2
• NCBI Official Synonym Symbols: LOR2; WS9-14
• NCBI Protein Information: lysyl oxidase homolog 2
• UniProt Protein Name: Lysyl oxidase homolog 2
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL2
• UniProt Entry Name: LOXL2_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Oxidoreductase; EC 1.4.3.13

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: basement membrane; chromosome; extracellular space; membrane; nucleoplasm; nucleus

Molecular Function: chromatin binding; copper ion binding; electron carrier activity; methylated histone residue binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity

Biological Process: aging; cell adhesion; collagen fibril organization; endothelial cell migration; endothelial cell proliferation; epithelial to mesenchymal transition; histone modification; negative regulation of transcription, DNA-dependent; positive regulation of chondrocyte differentiation; protein amino acid deamination; protein modification process; receptor-mediated endocytosis; response to copper ion; response to hypoxia; sprouting angiogenesis; transcription, DNA-dependent