Lactoferrin/LTF ELISA Pair Set | LTF elisa pair set

Lactoferrin/LTF Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118351

• Synonyms: Lactoferrin/LTF; Lactoferrin/LTF Matched ELISA Antibody Pair Set; Human; Human Lactotransferrin/LTF ELISA Pair Set; lactotransferrin; GIG12 Matched ELISA Antibody Pair Set; HEL110 Matched ELISA Antibody Pair Set; HLF2 Matched ELISA Antibody Pair Set; LF Matched ELISA Antibody Pair Set; LTF elisa pair set

• Specificity: Quantitative determination of Human Lactoferrin/LTF

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 78.125pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human Lactotransferrin/LTF monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Lactotransferrin/LTF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 180 ng of recombinant Human Lactotransferrin/LTF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for LTF elisa pair set

Lactotransferrin, also known as Lactoferrin, Talalactoferrin, and LTF, is a secreted protein that belongs to the transferrin family. Transferrins are iron binding transport proteins that can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B, and C have opioid antagonist activity. Lactoferrin A shows a preference for mu-receptors, while lactoferricin B and lactoferricin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin/LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin/LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin/LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin/LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucose. In particular, lactoferrin provides an antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.

This Lactoferrin/LTF Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Lactoferrin/LTF. It contains Human Lactoferrin/LTF capture antibody, Human Lactoferrin/LTF detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Lactoferrin/LTF protein.

References

Sanchez L, et al., 1992, Arch. Dis. Child. 67 (5): 657-61. Wakabayashi H, et al., 2000, J. Antimicrob. Chemother. 46 (4): 595-602. Nozaki A, et al., 2003, J. Biol. Chem. 278 (12): 10162-73. Azzam HS, et al., 2007, Liver Int. 27 (1): 17-25.