Alpha 2 Antiplasmin/SerpinF2 ELISA Pair Set | SerpinF2 elisa pair set

Alpha 2 Antiplasmin/SerpinF2 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118229

• Synonyms: Alpha 2 Antiplasmin/SerpinF2; Alpha 2 Antiplasmin/SerpinF2 Matched ELISA Antibody Pair Set; Human; Human SerpinF2 ELISA Pair Set; serpin peptidase inhibitor; clade F; member 2; A2AP Matched ELISA Antibody Pair Set; AAP Matched ELISA Antibody Pair Set; ALPHA-2-PI Matched ELISA Antibody Pair Set; API Matched ELISA Antibody Pair Set; PLI Matched ELISA Antibody Pair Set; SerpinF2 elisa pair set

• Specificity: Quantitative determination of Human Alpha 2 Antiplasmin/SerpinF2

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 31.25pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human SerpinF2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human SerpinF2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 195 ng of recombinant Human SerpinF2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for SerpinF2 elisa pair set

SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N-and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin(ogen). It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.

This Alpha 2 Antiplasmin/SerpinF2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Alpha 2 Antiplasmin/SerpinF2. It contains Human Alpha 2 Antiplasmin/SerpinF2 capture antibody, Human Alpha 2 Antiplasmin/SerpinF2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Alpha 2 Antiplasmin/SerpinF2 protein.

References

Lee KN, et al. (2004) Alpha2-antiplasmin: potential therapeutic roles in fibrin survival and removal. Curr Med Chem Cardiovasc Hematol Agents. 2(4): 303-10.Matsuno H. (2006) Alpha2-antiplasmin on cardiovascular diseases. Curr Pharm Des. 12(7): 841-7.Burnouf T, et al. (2007) Impact of Triton X-100 on alpha 2-antiplasmin (SERPINF2) activity in solvent/detergent-treated plasma. Biologicals. 35(4): 349-53.Carpenter SL, et al. (2008) Alpha2-antiplasmin and its deficiency: fibrinolysis out of balance. Haemophilia. 14(6): 1250-4.