SDS-Page
Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) Recombinant Protein | ALPPL2 recombinant protein
Recombinant Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2)
Gene Names
ALPPL2; ALPG; GCAP; ALPPL
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Alkaline Phosphatase; Placental Like Protein 2 (ALPPL2); Recombinant Alkaline Phosphatase; ALPPL2 recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 120ug/mL (varies by lot)
Sequence
I IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGR
Applicable Applications for ALPPL2 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Ile20~Arg333
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
6.9
Usage
Reconstitute in 20mM Tris, 150mM NaCI (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 35.8kDa
Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
alkaline phosphatase, placental-like preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental-like 2
NCBI Official Symbol
ALPPL2
NCBI Official Synonym Symbols
ALPG; GCAP; ALPPL
NCBI Protein Information
alkaline phosphatase, placental-like; ALP-1; Nagao isozyme; germ cell alkaline phosphatase; alkaline phosphatase Nagao isozyme; placental-like alkaline phosphatase; testicular and thymus alkaline phosphatase
UniProt Protein Name
Alkaline phosphatase, placental-like
Protein Family
UniProt Gene Name
ALPPL2
UniProt Synonym Gene Names
ALPPL; GCAP; PLAP-like
UniProt Entry Name
PPBN_HUMAN
NCBI Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]
Uniprot Description
ALPPL2: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]
Protein type: Phosphatase (non-protein); EC 3.1.3.1; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - folate biosynthesis
Chromosomal Location of Human Ortholog: 2q37
Cellular Component: plasma membrane
Molecular Function: alkaline phosphatase activity; metal ion binding
Biological Process: dephosphorylation
Research Articles on ALPPL2
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Product Notes
The ALPPL2 alppl2 (Catalog #AAA2011658) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ALPPL2 alppl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: I IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGR. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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