G-CSF ELISA Pair Set | CSF3 elisa pair set

G-CSF Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118171

• Synonyms: G-CSF; G-CSF Matched ELISA Antibody Pair Set; Human; Human G-CSF ELISA Pair Set; colony stimulating factor 3 (granulocyte); C17orf33 Matched ELISA Antibody Pair Set; C17orf33OS Matched ELISA Antibody Pair Set; CSF3OS Matched ELISA Antibody Pair Set; GCSF Matched ELISA Antibody Pair Set; CSF3 elisa pair set

• Specificity: Quantitative determination of Human G-CSF

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 93.75-6000pg/mL
• Sensitivity: 93.75pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human G-CSF polyclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating.2. Detection Antibody 0.2 mg/mL of rabbit anti-Human G-CSF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.25ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 305 ng of recombinant Human G-CSF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 6000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CSF3 elisa pair set

Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.

This G-CSF Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human G-CSF. It contains Human G-CSF capture antibody, Human G-CSF detector antibody (HRP) and a highly purified recombinant Human G-CSF protein.

Product Categories/Family for CSF3 elisa pair set

IL17 signaling pathway

References

Takano H, et al. (2007) G-CSF therapy for acute myocardial infarction. Trends Pharmacol Sci. 28(10): 512-7.
Klocke R, et al. (2008) Granulocyte colony-stimulating factor (G-CSF) for cardio-and cerebrovascular regenerative applications. Curr Med Chem. 15(10): 968-77.
Kang HJ, et al. (2008) G-CSF-and erythropoietin-based cell therapy: a promising strategy for angiomyogenesis in myocardial infarction. Expert Rev Cardiovasc Ther. 6(5): 703-13.
Beekman R, et al. (2010) G-CSF and its receptor in myeloid malignancy. Blood. 115(25): 5131-6.