Testing Data
Goat anti-Any Nitrotyrosine Polyclonal Antibody
Goat Anti-Nitrotyrosine Polyclonal Antibody
Reactivity
Any
Applications
Immunoblot, Western Blot, ELISA
Purity
Affinity Purified
Synonyms
Nitrotyrosine; Polyclonal Antibody; Goat Anti-Nitrotyrosine Polyclonal Antibody; anti-Nitrotyrosine antibody
Host
Goat
Reactivity
Any
Clonality
Polyclonal
Specificity
Nitrated proteins
Purity/Purification
Affinity Purified
Form/Format
100 ug of affinity purified antibody at 1 mg/mL in 75 mM PBS, pH 7.2, 0.5 mM EDTA and 0.02% NaN3
Applicable Applications for anti-Nitrotyrosine antibody
Immunoblot (IB), ELISA (EIA)
Application Notes
Immunoblot (1:200 to 1:40,000)
ELISA (1:200 to 1:40,000)
ELISA (1:200 to 1:40,000)
Immunogen
Nitrated KLH
Conjugation
Unconjugated
Quantity
100 ug of affinity purified antibody at 1 mg/mL in 75 mM PBS, pH 7.2, 0.5 mM EDTA and 0.02% NaN3
Preparation and Storage
-20 degree C. Shelf Life: 1 year from date of receipt under proper storage conditions; aliquot to avoid multiple freeze thaw cycles
Testing Data
Testing Data
Related Product Information for anti-Nitrotyrosine antibody
• Affinity purified antibodies at 1 mg/mL
• Suitable for Western blot or ELISA applications
The modification of tyrosine residues in proteins to 3-nitrotyrosine by peroxynitrite (Figure 1) or other potential nitrating agents has been detected in biological systems that are subject to oxidative stress. Detection of nitrotyrosine-containing proteins has been reported in many human and animal diseases or cellular models of disease. While all tyrosine residues in proteins may theoretically be targets for nitration, presumably the efficiency of tyrosine nitration is dependent on various biological conditions such as the local production and concentration of the reactive species, the existence and availability of antioxidants and scavengers, the accumulation of inflammatory cell and the presence of pro-inflammatory cytokines, as well as the proximity and compartmentation of these components.
• Suitable for Western blot or ELISA applications
The modification of tyrosine residues in proteins to 3-nitrotyrosine by peroxynitrite (Figure 1) or other potential nitrating agents has been detected in biological systems that are subject to oxidative stress. Detection of nitrotyrosine-containing proteins has been reported in many human and animal diseases or cellular models of disease. While all tyrosine residues in proteins may theoretically be targets for nitration, presumably the efficiency of tyrosine nitration is dependent on various biological conditions such as the local production and concentration of the reactive species, the existence and availability of antioxidants and scavengers, the accumulation of inflammatory cell and the presence of pro-inflammatory cytokines, as well as the proximity and compartmentation of these components.
Product Categories/Family for anti-Nitrotyrosine antibody
Similar Products
Product Notes
The Nitrotyrosine (Catalog #AAA168713) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Nitrotyrosine Polyclonal Antibody reacts with Any and may cross-react with other species as described in the data sheet. AAA Biotech's Nitrotyrosine can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), ELISA (EIA). Immunoblot (1:200 to 1:40,000) ELISA (1:200 to 1:40,000). Researchers should empirically determine the suitability of the Nitrotyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitrotyrosine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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