Barbiturates ELISA Kit
Barbiturates ELISA Kit
Synonyms
Barbiturates; Barbiturates ELISA Kit; Barbiturates elisa kit
Specificity
This ELISA is specific for the measurement of natural and recombinant human BAFF. It does not cross-react with mouse BAFF. Detection of BAFF (human) in biological fluids by this ELISA kit is abolished in the presence of a BAFF receptor such as TACI:Fc
Samples
Serum, Plasma And Cell Culture Supernatant
Assay Type
Sandwich
Detection Range
15.6 pg/ml-500 pg/ml
Sensitivity
8 pg/ml
Intra-assay Precision
Five samples of known concentrations of human BAFF were assayed in replicates 16 times to test precision within an assay
Inter-assay Precision
Four samples of known concentrations of human BAFF were assayed in 4 separate assays to test precision between assays.
Related Product Information for Barbiturates elisa kit
Intended Uses: The BAFF, soluble (human) ELISA kit (hypersensitive) is to be used for the measurement of human BAFF in serum, plasma and cell culture supernatant. This ELISA Kit is for research use only.
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human BAFF (BAFF) in serum, plasma and cell culture supernatant. A monoclonal antibody specific for BAFF has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, BAFF is recognized by the addition of a biotinylated monoclonal antibody specific for BAFF (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of BAFF in the samples!!Background/Introduction: BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a key survival factor for peripheral B cells. BAFF is a homotrimeric type II transmembrane protein that can be proteolytically processed by furin to be released as soluble cytokine (1). Soluble BAFF adopts the classical trimeric form of the TNF-family ligand. However, BAFF has the unique property among the TNF-ligand to assemble as a 60-mer (2). BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation (1). Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of persons with various autoimmune diseases (3), such as Sjögren's syndrome (4), Rheumatoid Arthritis (RA) (5), Multiple sclerosis (MS) (6) and Systemic Lupus Erythematosus (SLE) (7). BAFF is also increased levels in some lymphoid cancers (8).
Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human BAFF (BAFF) in serum, plasma and cell culture supernatant. A monoclonal antibody specific for BAFF has been pre-coated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, BAFF is recognized by the addition of a biotinylated monoclonal antibody specific for BAFF (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of BAFF in the samples!!Background/Introduction: BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a key survival factor for peripheral B cells. BAFF is a homotrimeric type II transmembrane protein that can be proteolytically processed by furin to be released as soluble cytokine (1). Soluble BAFF adopts the classical trimeric form of the TNF-family ligand. However, BAFF has the unique property among the TNF-ligand to assemble as a 60-mer (2). BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation (1). Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of persons with various autoimmune diseases (3), such as Sjögren's syndrome (4), Rheumatoid Arthritis (RA) (5), Multiple sclerosis (MS) (6) and Systemic Lupus Erythematosus (SLE) (7). BAFF is also increased levels in some lymphoid cancers (8).
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Product Notes
The Barbiturates (Catalog #AAA580058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Barbiturates, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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