SDS-Page
(Recombinant DmTet (1634-1986, 2601- 2708) protein 12.5% SDS-PAGE gel with Coomassie blue staining MW: 53.68 kDa Purity: >70%)
DmTet recombinant protein
Recombinant DmTet (1634-1986, 2601-2708) protein
Synonyms
DmTet; Recombinant DmTet (1634-1986; 2601-2708) protein; Drosophila melanogaster Tet-likeprotein; DmTet recombinant protein
Host
E Coli
Form/Format
Recombinant DmTet (1634-1986 and 2601-2708) protein is supplied in 25mM Tris pH7.4, 300mM NaCl, 10% glycerol, and 0.5mM TCEP.
Protein Species
Drosophila melanogaster
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant DmTet (1634-1986, 2601- 2708) protein 12.5% SDS-PAGE gel with Coomassie blue staining MW: 53.68 kDa Purity: >70%)
SDS-Page
(Recombinant DmTet (1634-1986, 2601- 2708) protein 12.5% SDS-PAGE gel with Coomassie blue staining MW: 53.68 kDa Purity: >70%)
Testing Data
(Recombinant DmTet (1634-1986, 2601-2708) activity assay 500 nM of 30 nt ssDNA oligos with a 5mCpG site were incubated with 0 ug, 0.625 ug, 1.25 ug, 2.5 ug or 5 ug DmTet (1634-1986 and 2601-2708), respectively, in a 20 ul reaction system at 34 degree C for 1 hour. Sample was concentrated to 10 ul, and 1 ul was spotted onto nylon membrane. Anti-5hmC Ab and anti-5CaC Ab were used to detect products. DmTet (1634-1986 and 2601-2708) activity assay was detected by Dot-Blot.)
Testing Data
(Recombinant DmTet (1634-1986, 2601-2708) activity assay 500 nM of 30 nt ssDNA oligos with a 5mCpG site were incubated with 0 ug, 0.625 ug, 1.25 ug, 2.5 ug or 5 ug DmTet (1634-1986 and 2601-2708), respectively, in a 20 ul reaction system at 34 degree C for 1 hour. Sample was concentrated to 10 ul, and 1 ul was spotted onto nylon membrane. Anti-5hmC Ab and anti-5CaC Ab were used to detect products. DmTet (1634-1986 and 2601-2708) activity assay was detected by Dot-Blot.)
Related Product Information for DmTet recombinant protein
Background: Ten Eleven Translocation (TET) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation.
Product Categories/Family for DmTet recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of DmTet (1634-1986 and 2601-2708) is 53.68kDa
Similar Products
Product Notes
The DmTet (Catalog #AAA389421) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DmTet, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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