SDS-Page
(Recombinant TET1 (1418-2136) protein gel. Recombinant TET1 (1418-2136) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 84.3 kDa Purity:> 80%)
Tet1 recombinant protein
Recombinant Tet1 (1418-2136) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TET1 (1418-2136) protein gel. Recombinant TET1 (1418-2136) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 84.3 kDa Purity:> 80%)
SDS-Page
(Recombinant TET1 (1418-2136) protein gel. Recombinant TET1 (1418-2136) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 84.3 kDa Purity:> 80%)
Dot Blot (DB)
(Dot Blot analysis for recombinant TET1 (1418-2136) protein.M/b> 0.5 uM of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 (-), 125 (+), 250 (++) and 500 (+++) ng TET1(1418-2136) protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at RT for 1 hour. Sample was concentrated to 5 ul, and a 2 ul sample was spotted onto positively charged nitrocellulose membrane. 5hmC antibody was used to detect the generation of products.)
Dot Blot (DB)
(Dot Blot analysis for recombinant TET1 (1418-2136) protein.M/b> 0.5 uM of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 (-), 125 (+), 250 (++) and 500 (+++) ng TET1(1418-2136) protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at RT for 1 hour. Sample was concentrated to 5 ul, and a 2 ul sample was spotted onto positively charged nitrocellulose membrane. 5hmC antibody was used to detect the generation of products.)
Background: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET1 preferentially binds to CpG-rich sequences at the promoters of both transcriptionally active and Polycomb-repressed genes and modulates gene silencing induced by cytosine methylation by controlling the levels of 5-mC and 5-hmC at gene promoters. TET1 may have the dual function of repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. TET1 is important for stem cell maintenance and specification and is involved in the balance between pluripotency and lineage commitment. TET1 plays an important role in regulating adult hippocampal neurogenesis and cognition
NCBI and Uniprot Product Information
Uniprot Description
TET1: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC). Might initiate a process leading to cytosine demethylation through deamination into 5- hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'. Belongs to the TET family.
Protein type: Oxidoreductase; DNA-binding; EC 1.14.11.n2
Chromosomal Location of Human Ortholog: 10q21
Cellular Component: nucleus
Molecular Function: structure-specific DNA binding; zinc ion binding; iron ion binding
Biological Process: protein amino acid O-linked glycosylation; inner cell mass cell differentiation; transcription, DNA-dependent; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; gene expression; chromatin modification; regulation of gene expression, epigenetic
Research Articles on Tet1
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Product Notes
The Tet1 tet1 (Catalog #AAA388135) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tet1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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