SETMAR recombinant protein

Recombinant SETMAR protein

Cat. Num. AAA388168

• Gene Names: SETMAR; Mar1; METNASE
• Synonyms: SETMAR; Recombinant SETMAR protein; Mar1; HsMar1; METNASE; SETMAR recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant SETMAR protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant SETMAR protein gel. SETMAR protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 79 kDa Purity: >90%)

Related Product Information for SETMAR recombinant protein

Short Description: Recombinant SETMAR (accession number NP_006506.3) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 79 kDa. SETMAR (SET Domain and mariner transposase fusion gene) is a ubiquitously expressed nuclear fusion protein that contains an N-terminal SET domain with histone methyltransferase activity and a C-terminal mariner (MAR) transposase domain that recognizes and binds DNA. The gene exists as a fusion gene only in anthropoid primates; other organisms lack mariner transposase domain. SETMAR binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The mariner transposase domain recognizes and binds the 19-mer core of the 5'-TIR (terminal inverted repeats) of the Hsmar1 element. The SET domain specifically methylates lysines 4 and 36 of histone H3 which are epigenetic marks associated with transcriptional activation. SETMAR demonstrates in vivo end joining activity and may mediate genomic integration of foreign DNA. Recombinant SETMAR can be used to study enzyme kinetics and perform inhibitor screening assays.

Background: SETMAR (SET Domain and mariner transposase fusion gene) is a ubiquitously expressed nuclear fusion protein that contains an N-terminal SET domain with histone methyltransferase activity and a C-terminal mariner (MAR) transposase domain that recognizes and binds DNA. The gene exists as a fusion gene only in anthropoid primates; other organisms lack mariner transposase domain. SETMAR binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The mariner transposase domain recognizes and binds the 19-mer core of the 5'-TIR (terminal inverted repeats) of the Hsmar1 element. The SET domain specifically methylates lysines 4 and 36 of histone H3 which are epigenetic marks associated with transcriptional activation. SETMAR demonstrates in vivo end joining activity and may mediate genomic integration of foreign DNA.

Product Categories/Family for SETMAR recombinant protein

Recombinant Proteins; Histone Methyltransferase (HMT)

NCBI and Uniprot Product Information

• NCBI GI #: 194306650
• NCBI GeneID: 6419
• NCBI Accession #: NP_006506.3
• NCBI GenBank Nucleotide #: NM_006515.3
• Molecular Weight: 62,124 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETMAR isoform 1
• NCBI Official Synonym Full Names: SET domain and mariner transposase fusion gene
• NCBI Official Symbol: SETMAR
• NCBI Official Synonym Symbols: Mar1; METNASE
• NCBI Protein Information: histone-lysine N-methyltransferase SETMAR
• Protein Family: Histone-lysine N-methyltransferase

NCBI Description

This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]