SDS-Page
(Recombinant TET2 (1129-2002) protein gel. Recombinant TET2 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 102.7 kDa Purity: >45%)
TET2 recombinant protein
Recombinant TET2 (1129-2002) protein
Gene Names
TET2; MDS; KIAA1546
Synonyms
TET2; Recombinant TET2 (1129-2002) protein; MDS; KIAA1546; TET2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant TET2 (1129-2002) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TET2 (1129-2002) protein gel. Recombinant TET2 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 102.7 kDa Purity: >45%)
SDS-Page
(Recombinant TET2 (1129-2002) protein gel. Recombinant TET2 was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 102.7 kDa Purity: >45%)
Testing Data
(Recombinant TET2 (1129-2002) activity assay 500 nM of 30 bp dsDNA oligos (sequence: 5’-CAGTAACTGTGGTC/5mC/GGTAACTGACTTGCA-3’) were incubated with different concentrations of TET2 (1129-2002) protein in a 20 ?l reaction system containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 100 ?M ascorbate, 100 ?M alpha-ketoglutarate, 100 ?M ATP, 1 mM TCEP at room temperature for 2 hours. 20 ?l reaction product was concentrated to 50 ul, then 1 ?l product was spotted onto positively charged nylon membrane. 5hmC antibody was used to detect the generation of products.)
Testing Data
(Recombinant TET2 (1129-2002) activity assay 500 nM of 30 bp dsDNA oligos (sequence: 5’-CAGTAACTGTGGTC/5mC/GGTAACTGACTTGCA-3’) were incubated with different concentrations of TET2 (1129-2002) protein in a 20 ?l reaction system containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 100 ?M ascorbate, 100 ?M alpha-ketoglutarate, 100 ?M ATP, 1 mM TCEP at room temperature for 2 hours. 20 ?l reaction product was concentrated to 50 ul, then 1 ?l product was spotted onto positively charged nylon membrane. 5hmC antibody was used to detect the generation of products.)
Related Product Information for TET2 recombinant protein
Short Description: Recombinant TET2 (1129-2002) protein that includes amino acids 1129-2002 of human TET2 protein (accession number NP_001120680.1) was expressed in Sf9 cells and contains an N-terminal 6xHis-tag and DYKDDDDK-Tag with a molecular weight of 102.7 kDa. The purity of the protein is >70% by SDS-PAGE. It is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET2 plays an important role in normal myelopoiesis. Somatic loss-of-function mutations in TET2 gene frequently occur in patients with myeloid malignancies. TET2 loss increased hematopoietic stem cell self-renewal and myeloid transformation. TET2 promotes histone O-GlcNAcylation during gene transcription in embryonic stem cells. In addition, TET2 can be recruited to chromatin by CXXC domain protein IDAX. IDAX downregulates TET2 protein by activating caspase pathway.
Background: TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET2 plays an important role in normal myelopoiesis. Somatic loss-of-function mutations in TET2 gene frequently occur in patients with myeloid malignancies. TET2 loss increased hematopoietic stem cell self-renewal and myeloid transformation. TET2 promotes histone O-GlcNAcylation during gene transcription in embryonic stem cells. In addition, TET2 can be recruited to chromatin by CXXC domain protein IDAX. IDAX downregulates TET2 protein by activating caspase pathway.
Product Categories/Family for TET2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,481 Da
NCBI Official Full Name
methylcytosine dioxygenase TET2 isoform a
NCBI Official Synonym Full Names
tet methylcytosine dioxygenase 2
NCBI Official Symbol
TET2
NCBI Official Synonym Symbols
MDS; KIAA1546
NCBI Protein Information
methylcytosine dioxygenase TET2
Protein Family
NCBI Description
The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Research Articles on TET2
Similar Products
Product Notes
The TET2 (Catalog #AAA388136) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TET2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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