AGO1 recombinant protein

Recombinant AGO1 protein

Cat. Num. AAA388226

• Gene Names: AGO1; Q99; EIF2C; EIF2C1; GERP95
• Purity: The purity of AGO1 protein is >80 % pure by SDS-PAGE.
• Synonyms: AGO1; Recombinant AGO1 protein; Argonaute 1; Argonaute1; RISC Catalytic Component; EIF-2C; EIF2C; EIF2C1; GERP95; Q99; AGO1 recombinant protein

• Host: Baculovirus
• Purity/Purification: The purity of AGO1 protein is >80 % pure by SDS-PAGE.
• Form/Format: Recombinant full length AGO1 protein was expressed in Sf9 cells and is provided in 25mM HEPES pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant AGO1 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling. This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant AGO1 protein gel. AGO1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 100.2 kDa Purity: > 85%)

Related Product Information for AGO1 recombinant protein

Short Description: Recombinant Human AGO1 was expressed in a baculovirus expression system as the full-length protein (accession number NP_036331.1) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 100.2 kDa. The purity of AGO1 protein is >75% pure by SDS-PAGE. Recombinant AGO1 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling.

Background: AGO1 (argonaute RISC catalytic component 1, also known as Q99; EIF2C1) is a member of the Argonaute family of proteins which play a role in RNA interference. The Argonaute family proteins (AGO1, AGO2, AGO3 and AGO4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleaves the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). AGO1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain.

Product Categories/Family for AGO1 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 6912352
• NCBI GeneID: 26523
• NCBI Accession #: NP_036331.1
• NCBI GenBank Nucleotide #: NM_012199.2
• UniProt Accession #: Q9UL18; Q5TA57; Q6P4S0
• Molecular Weight: 97,214 Da
• NCBI Official Full Name: protein argonaute-1
• NCBI Official Synonym Full Names: argonaute RISC catalytic component 1
• NCBI Official Symbol: AGO1
• NCBI Official Synonym Symbols: Q99; EIF2C; EIF2C1; GERP95
• NCBI Protein Information: protein argonaute-1; hAgo1; eIF2C 1; eIF-2C 1; argonaute1; argonaute 1; putative RNA-binding protein Q99; Golgi Endoplasmic Reticulum protein 95 kDa; eukaryotic translation initiation factor 2C, 1
• UniProt Protein Name: Protein argonaute-1
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO1
• UniProt Synonym Gene Names: EIF2C1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1
• UniProt Entry Name: AGO1_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4. [provided by RefSeq, Jul 2008]

Uniprot Description

AGO1: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA. Belongs to the argonaute family. Ago subfamily.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; polysome; cytoplasm; cytosol; ribonucleoprotein complex

Molecular Function: endoribonuclease activity; protein binding; miRNA binding; RNA binding

Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; innate immune response; gene expression; positive regulation of transcription from RNA polymerase II promoter; posttranscriptional gene silencing