SDS-Page
(Recombinant AGO2 protein gel. AGO2 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 105 kDa Purity: > 80%)
AGO2 recombinant protein
Recombinant AGO2 protein
Gene Names
AGO2; Q10; EIF2C2
Purity
The recombinant protein is > 85% pure by SDS-PAGE.
Synonyms
AGO2; Recombinant AGO2 protein; Q10; EIF2C2; AGO2 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is > 85% pure by SDS-PAGE.
Form/Format
Recombinant AGO2 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant AGO2 protein gel. AGO2 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 105 kDa Purity: > 80%)
SDS-Page
(Recombinant AGO2 protein gel. AGO2 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 105 kDa Purity: > 80%)
Related Product Information for AGO2 recombinant protein
Short Description: Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with an observed molecular weight of 104 kDa. The recombinant protein is >90% pure by SDS-PAGE.
Background: AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10; EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleave the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions.
Background: AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10; EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleave the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions.
Product Categories/Family for AGO2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,620 Da
NCBI Official Full Name
Homo sapiens argonaute RISC catalytic component 2 (AGO2), transcript variant 1, mRNA
NCBI Official Synonym Full Names
argonaute RISC catalytic component 2
NCBI Official Symbol
AGO2
NCBI Official Synonym Symbols
Q10; EIF2C2
NCBI Protein Information
protein argonaute-2; PAZ Piwi domain protein; PPD; argonaute 2; argonaute2; eIF-2C 2; eIF2C 2; eukaryotic translation initiation factor 2C, 2; hAgo2; protein slicer
Protein Family
NCBI Description
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Research Articles on AGO2
Similar Products
Product Notes
The AGO2 (Catalog #AAA388197) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AGO2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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