SDS-Page
(Recombinant CREBBP (1081-1197) protein gel. CREBBP (1081-1197) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.)
CREBBP recombinant protein
Recombinant CREBBP (1081-1197) protein
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
CREBBP; Recombinant CREBBP (1081-1197) protein; CBP; RSTS; KAT3A; CREBBP recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Recombinant CREBBP (1081-1197) protein is supplied in 25mM Tris pH8, 150mM NaCl and 10% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Protein Details
Recombinant CREBBP protein that includes amino acids 1081-1197 that contains the bromodomain sequences of CREBBP (accession number NM_004380.2) was expressed in E Coli and contains an N-terminal His-Tag and C-terminal DYKDDDDK-Tag with an observed molecular weight of 18.7kDa. It shows binding specificity for acetylated H2BK85, H3K9/K14, H3K14, H3K36, H3K56, H3S10/K14/K18, H4K12, H4K20 and H4K44, as well as acetylated p53-K382, MyoD-K99 and MyoD-K99/K102.
Notes
Recombinant CREBBP (1081-1197) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant CREBBP (1081-1197) protein gel. CREBBP (1081-1197) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant CREBBP (1081-1197) protein gel. CREBBP (1081-1197) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(Recombinant CREBBP (1081-1197) HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of CREBBP (1081-1197) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in reaction buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant CREBBP (1081-1197) HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of CREBBP (1081-1197) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in reaction buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for CREBBP recombinant protein
Short Description: The peptide corresponding to amino acids 1081-1197 that contains the bromodomain sequences of CREBBP (accession number NM_004380.2) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 18.7 kDa. It shows binding specificity for acetylated H2BK85, H3K9/K14, H3K14, H3K36, H3K56, H3S10/K14/K18, H4K12, H4K20 and H4K44, as well as acetylated p53-K382, MyoD-K99 and MyoD-K99/K102. CREB-binding protein (CREBBP) or CBP, is a protein known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. The CREBBP bromodomain recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. CREBBP has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. CREBBP acetylates histones as well as non-histone proteins, like NCOA3 coactivator. In addition, CREBBP binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. CREBBP also has been shown to act as a coactivator of ALX1 in the presence of p300. Recombinant CREBBP (1081-1197) can be used in binding assays and inhibitor screening.
Background: CREB-binding protein (CREBBP) or CBP, is a protein known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. The CREBBP bromodomain recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. CREBBP has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. CREBBP acetylates histones as well as non-histone proteins, like NCOA3 coactivator. In addition, CREBBP binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. CREBBP also has been shown to act as a coactivator of ALX1 in the presence of p300.
Background: CREB-binding protein (CREBBP) or CBP, is a protein known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. CREBBP shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. The CREBBP bromodomain recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. CREBBP has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. CREBBP acetylates histones as well as non-histone proteins, like NCOA3 coactivator. In addition, CREBBP binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. CREBBP also has been shown to act as a coactivator of ALX1 in the presence of p300.
Product Categories/Family for CREBBP recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 18.7 kDa
Protein Family
Similar Products
Product Notes
The CREBBP (Catalog #AAA388093) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CREBBP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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