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Western Blot (WB) (Western blotAll lanes: DNAJB5 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40,48,52 kDaObserved band size: 39 kDa)

Rabbit anti-Human DNAJB5 Polyclonal Antibody | anti-DNAJB5 antibody

DNAJB5 Antibody

Gene Names
DNAJB5; Hsc40
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
DNAJB5; Polyclonal Antibody; DNAJB5 Antibody; DnaJ homolog subfamily B member 5; Heat shock protein Hsp40-2; Heat shock protein Hsp40-3; Heat shock protein cognate 40; Hsc40; HSC40; anti-DNAJB5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
462
Applicable Applications for anti-DNAJB5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human DnaJ homolog subfamily B member 5 protrin
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: DNAJB5 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40,48,52 kDaObserved band size: 39 kDa)

Western Blot (WB) (Western blotAll lanes: DNAJB5 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateLane 3:Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40,48,52 kDaObserved band size: 39 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis using MBS7006340 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS7006340 at dilution 1:100)

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysateAll lanes: DNAJB5 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40,48,52 KDaObserved band size: 48 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: HepG2 whole cell lysateAll lanes: DNAJB5 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40,48,52 KDaObserved band size: 48 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human glioma cancer using MBS7006340 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of human glioma cancer using MBS7006340 at dilution of 1:100)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,689 Da
NCBI Official Full Name
dnaJ homolog subfamily B member 5 isoform 2
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member B5
NCBI Official Symbol
DNAJB5
NCBI Official Synonym Symbols
Hsc40
NCBI Protein Information
dnaJ homolog subfamily B member 5
UniProt Protein Name
DnaJ homolog subfamily B member 5
Protein Family
UniProt Gene Name
DNAJB5
UniProt Synonym Gene Names
HSC40; Hsc40
UniProt Entry Name
DNJB5_HUMAN

NCBI Description

DNAJB5 belongs to the evolutionarily conserved DNAJ/HSP40 protein family. For background information on the DNAJ family, see MIM 608375.[supplied by OMIM, Mar 2008]

Uniprot Description

DNAJB5: Expressed under normal conditions, its expression can further be increased after various stress treatments. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytosol; nucleus

Molecular Function: chaperone binding; protein binding; unfolded protein binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; protein folding; response to unfolded protein

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Product Notes

The DNAJB5 dnajb5 (Catalog #AAA7006340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJB5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the DNAJB5 dnajb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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