SDS-Page
(Recombinant BRWD3 (1295-1443) protein gel. BRWD3 (1295-1443) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 23.4 kDa Purity: > 80%)
BRWD3 recombinant protein
Recombinant BRWD3 (1295-1443) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BRWD3 (1295-1443) protein gel. BRWD3 (1295-1443) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 23.4 kDa Purity: > 80%)
SDS-Page
(Recombinant BRWD3 (1295-1443) protein gel. BRWD3 (1295-1443) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 23.4 kDa Purity: > 80%)
Testing Data
(Recombinant BRWD3 (1295-1443) HTRF activity assay. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRWD3 (1295-1443) in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 mixture (each at 1:100 dilution in reaction buffer) was added for 30 minutes . All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant BRWD3 (1295-1443) HTRF activity assay. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRWD3 (1295-1443) in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 mixture (each at 1:100 dilution in reaction buffer) was added for 30 minutes . All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: Bromodomain and WD repeat domain containing 3 (BRWD3) is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains a bromodomain and multiple WD repeats. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. Mutations in BRWD3 cause mental retardation X-linked type 93, which is also referred to as mental retardation X-linked with macrocephaly. This gene is also associated with translocations in patients with B-cell chronic lymphocytic leukemia (B-CLL).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains a bromodomain and several WD repeats. It is thought to have a chromatin-modifying function, and may thus play a role in transcription. Mutations in this gene cause mental retardation X-linked type 93, which is also referred to as mental retardation X-linked with macrocephaly. This gene is also associated with translocations in patients with B-cell chronic lymphocytic leukemia. [provided by RefSeq, May 2010]
Uniprot Description
BRWD3: Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. A chromosomal aberration involving BRWD3 can be found in patients with B-cell chronic lymphocytic leukemia (B- CLL). Translocation t(X;11)(q21;q23) with ARHGAP20 does not result in fusion transcripts but disrupts both genes. Defects in BRWD3 are the cause of mental retardation X- linked type 93 (MRX93); also known as mental retardation X-linked with macrocephaly. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Mentally retarded individuals are at least twice as likely to have macrocephaly than are their intellectually normal peers. 5 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: Xq21.1
Cellular Component: nucleus
Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of cell shape; cytoskeleton organization and biogenesis
Disease: Mental Retardation, X-linked 93
Research Articles on BRWD3
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Product Notes
The BRWD3 brwd3 (Catalog #AAA388157) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRWD3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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