CECR2 recombinant protein

Recombinant CECR2 (425-538) protein

Cat. Num. AAA388094

• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: CECR2; Recombinant CECR2 (425-538) protein; CECR2 recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Recombinant CECR2 (425-538) protein was expressed in E Coli cells and is supplied in 25mM Tris-HCl pH8.0, 500mM NaCl, 0.04% Triton X-100, 20% glycerol, 1mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant CECR2 (425-538) protein gel. CECR2 (425-538) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)

Testing Data

(Recombinant CECR2 (425-538) activity assay. 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with CECR2 (425-538) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)

Related Product Information for CECR2 recombinant protein

Short Description: The peptide corresponding to amino acids 425-538 that contains the bromodomain sequences of CECR2 (accession number NM_031413.3) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 19.7 kDa. CECR2 protein is the bromodomain-containing subunit of the CERF (CECR2-containing-remodeling factor) complex. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. The CERF complex functions to facilitate the perturbation of chromatin structure in an ATP-dependent manner. In addition to its chromatin remodeling function, CECR2 interaction with LRPPRC may be involved in the integration of the cytoskeletal network with processes such as vesicular trafficking, nucleocytosolic shuttling, transcription, chromosome remodeling and cytokinesis.

Background: CECR2 protein is the bromodomain-containing subunit of the CERF (CECR2-containing-remodeling factor) complex. Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. The CERF complex functions to facilitate the perturbation of chromatin structure in an ATP-dependent manner. In addition to its chromatin remodeling function, CECR2 interaction with LRPPRC may be involved in the integration of the cytoskeletal network with processes such as vesicular trafficking, nucleocytosolic shuttling, transcription, chromosome remodeling and cytokinesis.

Product Categories/Family for CECR2 recombinant protein

Recombinant Proteins; Bromodomain

NCBI and Uniprot Product Information

• NCBI GenBank Nucleotide #: NM_031413.3
• Molecular Weight: Molecular weight of 19.7kDa
• Protein Family: Cat eye syndrome critical region protein