SDS-Page
(Recombinant BRDT (21-137) protein, GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.2 kDa Purity: >80%)
BRDT recombinant protein
Recombinant BRDT (21-137) protein, GST-Tag
Shipping Temp: Dry Ice
SDS-Page
(Recombinant BRDT (21-137) protein, GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.2 kDa Purity: >80%)
SDS-Page
(Recombinant BRDT (21-137) protein, GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 40.2 kDa Purity: >80%)
Testing Data
(HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. It shows binding specificity for acetylated H2BK12/15, H3K9/14/18/23, H4K12, H4K5/8, H4K8/12, H4K12/16, H4K5/12, H4K5/8/12/16 and H4K5/8/12/16.
NCBI and Uniprot Product Information
NCBI Description
BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Uniprot Description
Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
Research Articles on BRDT
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Product Notes
The BRDT brdt (Catalog #AAA389213) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BRDT, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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