SDS-Page
(Recombinant TRIM33 (959-1069) protein gel. TRIM33 (959-1069) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.7 kDa Purity: >80%)
TRIM33 recombinant protein
Recombinant TRIM33 (959-1069) protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant TRIM33 (959-1069) protein gel. TRIM33 (959-1069) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.7 kDa Purity: >80%)
SDS-Page
(Recombinant TRIM33 (959-1069) protein gel. TRIM33 (959-1069) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 17.7 kDa Purity: >80%)
Testing Data
(Recombinant TRIM33 (959-1069) protein HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of TRIM33 (959- 1069) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant TRIM33 (959-1069) protein HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of TRIM33 (959- 1069) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant TRIM33 (959-1069) protein HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of TRIM33 (959- 1069) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant TRIM33 (959-1069) protein HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of TRIM33 (959- 1069) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: Tripartite motif-containing 33 (TRIM33) protein, also known as TIF1g, is a member of the transcriptional intermediary factor 1 (TIF1) family that control transcription and chromatin remodeling through their interaction with transcription factors. The family includes TRIM24 (TIF1a), TRIM28 (TIF1b) and TRIM33 (TIF1g) that share a characteristic domain structure comprised of multiple histone-binding domains, an N-terminal TRIM region (containing a RING domain, B box type 1 and type 2 domains, and a coiled-coil region), and a C-terminal bromodomain and PHD finger. Bromodomains function as 'readers' of epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TRIM33 is an E3 ubiquitin protein ligase that promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. TRIM33 does not affect SMAD4 levels but rather acts as an inhibitor of the SMAD4-dependent TGFb/BMP signaling cascade by monoubiquitinating SMAD4 and hampering its ability to form a stable complex with activated SMAD2/3. TRIM33 may also act as a transcriptional corepressor. It has also been shown to associate with SMAD2 and SMAD3 and stimulate differentiation of hematopoietic stem cells. Additionally, TRIM33 associates with TRIM24 and plays a role in the control of cell proliferation.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.
Protein type: Ubiquitin ligase; Transcription, coactivator/corepressor; EC 6.3.2.19; Protein kinase, atypical; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Kinase, protein; ATYPICAL group; TIF1 family
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway
Disease: Thyroid Carcinoma, Papillary
Research Articles on TRIM33
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Product Notes
The TRIM33 trim33 (Catalog #AAA388087) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TRIM33, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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