SDS-Page
(Recombinant p300 (1041-1161) protein gel. p300 (1041-1161) protein was run on a 15% SDS-PAGE gel and stained with Coomassie Blue.)
p300 recombinant protein
Recombinant p300 (1041-1161) protein
Purity
The recombinant protein is >90% pure by SDS-PAGE.
Synonyms
p300; Recombinant p300 (1041-1161) protein; EP300; KAT3B; RSTS2; p300 recombinant protein
Host
E Coli
Purity/Purification
The recombinant protein is >90% pure by SDS-PAGE.
Form/Format
Recombinant p300 (1041-1161) protein expressed in E Coli and supplied in 25mM Tris pH7.4, 150mM NaCl, 5% Glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Protein Details
The peptide corresponding to amino acids 1041-1161 that contains the bromodomain sequences of EP300 (accession number NM_001429.3) was expressed in E Coli and contains an N-terminal His-Tag and C-terminal DYKDDDDK-Tag with an observed molecular weight of 19.1kDa. It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102.
Notes
Recombinant p300 (1041-1161) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant p300 (1041-1161) protein gel. p300 (1041-1161) protein was run on a 15% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant p300 (1041-1161) protein gel. p300 (1041-1161) protein was run on a 15% SDS-PAGE gel and stained with Coomassie Blue.)
Testing Data
(Recombinant p300 (1041-1161) activity using AlphaScreen. p300 (1041-1161) titration and inhibition were assessed using an AlphaScreen assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
Testing Data
(Recombinant p300 (1041-1161) activity using AlphaScreen. p300 (1041-1161) titration and inhibition were assessed using an AlphaScreen assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
Related Product Information for p300 recombinant protein
Short Description: Recombinant p300 (1041-1161) protein was expressed in E Coli and contains an N-terminal His-Tag and C-terminal DYKDDDDK-Tag with an observed molecular weight of 19.1 kDa. This peptide, corresponding to amino acids 1041-1161, contains the bromodomain sequences of EP300 (accession number NM_001429.3). It shows binding specificity for acetylated H3K36, H3K56, H4K12, H4K20 and H4K44, as well as acetylated MyoD-K99/K102. E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation, and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought to regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and it regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets.
Background: E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Background: E1A binding protein p300 (EP300) or p300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates 'Lys131' of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TAT's transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a 'reader' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
Product Categories/Family for p300 recombinant protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The p300 (Catalog #AAA388092) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "p300, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
