SDS-Page
(Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel. SMARCA2 / BRM (1367-1511), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 44.6 kDa Purity: > 85%)
SMARCA2/BRM recombinant protein
Recombinant SMARCA2/BRM (1367-1511), GST-Tag
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel. SMARCA2 / BRM (1367-1511), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 44.6 kDa Purity: > 85%)
SDS-Page
(Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel. SMARCA2 / BRM (1367-1511), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. MW: 44.6 kDa Purity: > 85%)
Testing Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Testing Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Testing Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Testing Data
(Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour at room temperature. GST antibody and SA-XL665 mixture (each at 1:100 in the reaction buffer) was added for an additional 30 minutes. All operations were performed at RT. HTRF assay was used for detection.)
Background: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA2 contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). SMARCA1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). SMARCA2 plays a pivotal role in the regulation of the switch in subunit composition of the npBAF and nBAF complexes as cells transition from proliferating neural stem/progenitor cells to post-mitotic neurons during neural development.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]
Uniprot Description
SMARCA2: an enzyme with ATP-dependent DNA helicase activity. A transcriptional coactivator that cooperates with nuclear hormone receptors to potentiate transcriptional activation. Binds TOPBP1. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.
Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 9p24.3
Cellular Component: intermediate filament cytoskeleton; intracellular membrane-bound organelle; nBAF complex; npBAF complex; nuclear chromatin; nucleoplasm; nucleus; SWI/SNF complex
Molecular Function: ATP binding; chromatin binding; DNA-dependent ATPase activity; helicase activity; histone binding; protein binding; transcription coactivator activity; transcription regulatory region DNA binding
Biological Process: chromatin remodeling; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; spermatid development; transcription from RNA polymerase II promoter
Disease: Nicolaides-baraitser Syndrome
Research Articles on SMARCA2/BRM
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Product Notes
The SMARCA2/BRM smarca2 (Catalog #AAA388192) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SMARCA2/BRM, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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