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SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

CD39L2/ENTPD6 recombinant protein

Recombinant mouse CD39L2/ENTPD6 Protein

Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
CD39L2/ENTPD6; Recombinant mouse CD39L2/ENTPD6 Protein; ectonucleoside triphosphate diphosphohydrolase 6 isoform1; 2700026H11Rik; Cd39l; Cd39l2; dJ738P15.3; NTPDa; NTPDase-6; Entpd6; CD39L2/ENTPD6 recombinant protein
Ordering
For Research Use Only!
Host
HEK293
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 20% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
33-455aa
Sequence
KWHRAS AAQAFFTIAG AASGARWTQQ AFSSPGSAAR GHEVFYGIMF DAGSTGTRIH VFQFARPPGE TPTLTHETFK ALKPGLSAYA DDVEKSAQGI QELLNVAKQH IPYDFWKATP LVLKATAGLR LLPGEKAQKL LQKVKEVFKA SPFLVGDDCV SIMNGTDEGV SAWITVNFLT GSLKTPGSSS VGMLDLGGGS TQITFLPRVE GTLQASPPGH LTALQMFNRT YKLYSYSYLG LGLMSARLAI LGGVEGKPAE NDKELVSPCL SPRFRGEWEH AEVTYRISGQ KAVGLYELCA SRVSEVLRNK VHRTEEAQHV DFYAFSYYYD LAASFGLIDA EKGGSLVVGD FEIAAKYVCR TLETQPPSSP FACMDLTYIS LLLHEFGFPG DKVLKLARKI DNVETSWALG AIFHYIDSLK RQKVPAL
Applicable Applications for CD39L2/ENTPD6 recombinant protein
SDS-PAGE, Enzyme Activity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
His-Tag
Predicted N-term
Asp
Bioactivity
Specific activity is > 80,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze GDP per minute at pH7.5 at 25 degree C.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for CD39L2/ENTPD6 recombinant protein
CD39L2, also known as ENTPD6, is a nucleoside phosphohydrolase of the CD39 family of enzymes that is present on the surface of cells. It is soluble monomeric enzyme, making it more amenable to thorough structural and fuctional analysis than the membrane-bound forms. Soluble CD39L2 preferentially exhibits nucleoside diphosphatase activity, which is depedent on the presence of divalent cations. It is expressed at its highest levels in cardiac muscle and in heart capillary endothelial cells. Also, it plays a role in the regulation and recruitment of platelet activation in heart. Recombinant mouse CD39L2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD39L2/ENTPD6 recombinant protein
References
Yeung G., et al, (2000) Biochemistry. 39:12916-12923. Hicks-Berger CA, et al, (2000) J Biol Chem. 275:34041-34045. Ivanenkov VV, et al, (2003) Biochemistry. 42:11726-11735.

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
47.3kDa (433aa)

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Product Notes

The CD39L2/ENTPD6 (Catalog #AAA201297) is a Recombinant Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 33-455aa. AAA Biotech's CD39L2/ENTPD6 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Enzyme Activity. Researchers should empirically determine the suitability of the CD39L2/ENTPD6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KWHR AS AAQAFFTIAG AASGARWTQQ AFSSPGSAAR GHEVFYGIMF DAGSTGTRIH VFQFARPPGE TPTLTHETFK ALKPGLSAYA DDVEKSAQGI QELLNVAKQH IPYDFWKATP LVLKATAGLR LLPGEKAQKL LQKVKEVFKA SPFLVGDDCV SIMNGTDEGV SAWITVNFLT GSLKTPGSSS VGMLDLGGGS TQITFLPRVE GTLQASPPGH LTALQMFNRT YKLYSYSYLG LGLMSARLAI LGGVEGKPAE NDKELVSPCL SPRFRGEWEH AEVTYRISGQ KAVGLYELCA SRVSEVLRNK VHRTEEAQHV DFYAFSYYYD LAASFGLIDA EKGGSLVVGD FEIAAKYVCR TLETQPPSSP FACMDLTYIS LLLHEFGFPG DKVLKLARKI DNVETSWALG AIFHYIDSLK RQKVPAL. It is sometimes possible for the material contained within the vial of "CD39L2/ENTPD6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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