BLMH active protein

Recombinant BLMH

Cat. Num. AAA201145

• Gene Names: Blmh; Bh; Bmh; AI035728
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: BLMH; Recombinant BLMH; Bmh; Bh; Blm hydrolase; bleomycin hydrolase; AI035728; Bleomycin hydrolase; Blmh; EC 3.4.22.40; BLMH active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 20mM Tris-HCl (pH8.0) Containing 1mM DTT, 0.1M NaCl, 30% Glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 1-455aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MGS MNNAGLN SEKVSALIQK LNSDPQFVLA QNVGTTHDLL DICLRRATVQ GAQHVFQHVV PQEGKPVTNQ KSSGRCWIFS CLNVMRLPFM KKFNIEEFEF SQSYLFFWDK VERCYFFLNA FVDTAQKKEP EDGRLVQYLL MNPTNDGGQW DMLVNIVEKY GVVPKKCFPE SHTTEATRRM NDILNHKMRE FCIRLRNLVH SGATKGEISS TQDAMMEEIF RVVCICLGNP PETFTWEYRD KDKNYHKIGP ITPLQFYKEH VKPLFNMEDK ICFVNDPRPQ HKYNKLYTVD YLSNMVGGRK TLYNNQPIDF LKKMVAASIK DGEAVWFGCD VGKHFNGKLG LSDMNVYDHE LVFGVSLKNM NKAERLAFGE SLMTHAMTFT AVSEKDNQEG TFVKWRVENS WGEDHGHKGY LCMTDEWFSE YVYEVVVDKK HVPEEVLAVL EQEPIVLPAW DPMGALAE
• Sequence Length: 455

• Applicable Applications for BLMH active protein: SDS-PAGE, Enzyme Activity
• Species: Mouse
• Tag: His-Tag
• Biological Activity: Specific activity is > 1,500 pmole/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for BLMH active protein

BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug Blm. It catalyzes the inactivation of the antitumor drug Blm (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant mouse BLMH protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for BLMH active protein

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 30519997
• NCBI GeneID: 104184
• NCBI Accession #: NP_848760.1
• NCBI GenBank Nucleotide #: NP_848760.1
• UniProt Accession #: Q8R016
• Molecular Weight: 54.9 kDa (478aa), confirmed by MALDI-TOF
• NCBI Official Full Name: bleomycin hydrolase
• NCBI Official Synonym Full Names: bleomycin hydrolase
• NCBI Official Symbol: Blmh
• NCBI Official Synonym Symbols: Bh; Bmh; AI035728
• NCBI Protein Information: bleomycin hydrolase
• UniProt Protein Name: Bleomycin hydrolase
• Protein Family: Bleomycin hydrolase
• UniProt Gene Name: Blmh
• UniProt Synonym Gene Names: BH; BLM hydrolase; BMH

NCBI Description

The encoded protein is a cytoplasmic cysteine peptidase involved in inactivation of bleomycin, a glycopeptide which is a component of combination chemotherapy regimens for cancer. This encoded enzyme is highly conserved, and it contains the signature active site residues of cysteine protease papain superfamily enzymes. It is postulated that this enzyme has protective effects against bleomycin-induced pulmonary fibrosis and bleomycin tumor resistance. [provided by RefSeq, Jan 2010]

Uniprot Description

BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.40; Protease

Chromosomal Location of Human Ortholog: 11|11 B5

Cellular Component: cytoplasm; nucleus

Molecular Function: aminopeptidase activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; identical protein binding; peptidase activity

Biological Process: homocysteine catabolic process; proteolysis; response to drug; response to toxin