Carbohydrate sulfotransferase 3/CHST3 Recombinant Protein | CHST3 recombinant protein

Recombinant human Carbohydrate sulfotransferase 3/CHST3 protein

Cat. Num. AAA201314

• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Carbohydrate sulfotransferase 3/CHST3; Recombinant human Carbohydrate sulfotransferase 3/CHST3 protein; Carbohydrate sulfotransferase 3; CHST3; CHST-3; Chondroitin 6-O-sulfotransferase 1; C6ST; C6ST1; C6ST-1; C6ST1HSD; Chondroitin 6-sulfotransferase; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0; GST-0; HSD; carbohydrate (chondroitin 6) sulfotransferase 3; CHST3 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 39-479aa
• Sequence: EKENKII SRVSDKLKQI PQALADANST DPALILAENA SLLSLSELDS AFSQLQSRLR NLSLQLGVEP AMEAAGEEEE EQRKEEEPPR PAVAGPRRHV LLMATTRTGS SFVGEFFNQQ GNIFYLFEPL WHIERTVSFE PGGANAAGSA LVYRDVLKQL FLCDLYVLEH FITPLPEDHL TQFMFRRGSS RSLCEDPVCT PFVKKVFEKY HCKNRRCGPL NVTLAAEACR RKEHMALKAV RIRQLEFLQP LAEDPRLDLR VIQLVRDPRA VLASRMVAFA GKYKTWKKWL DDEGQDGLRE EEVQRLRGNC ESIRLSAELG LRQPAWLRGR YMLVRYEDVA RGPLQKAREM YRFAGIPLTP QVEDWIQKNT QAAHDGSGIY STQKNSSEQF EKWRFSMPFK LAQVVQAACG PAMRLFGYKL ARDAAALTNR SVSLLEERGT FWVT

• Applicable Applications for CHST3 recombinant protein: SDS-PAGE, Enzyme Activity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Specific activity is > 1,000pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to Chondroitin Sulfate per minute at pH7.5, at 25 degree C.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CHST3 recombinant protein

CHST3, also known as chondroitin 6-O-sulfotransferase, is a member of sulfotransferase 1 family. The human CHST family is comprised of 14 enzymes and all members of this family are Golgi-localized type II membrane proteins. These enzymes utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. It can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. CHST3 Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix. Recombinant human CHST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CHST3 recombinant protein

Signal Transduction; Cytoskeleton; Proteins

References

Hemmerich, S. and Rosen, S. Glycobiology 10:849-856. Uchimura, K. et al. J. Biol. Chem.277:1443-1450. Yusa, A. et al. J. Biol. Chem. 281:20393-20403.

NCBI and Uniprot Product Information

• UniProt Accession #: Q7LGC8
• Molecular Weight: 51.3kDa (450aa)
• UniProt Protein Name: Carbohydrate sulfotransferase 3
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST3
• UniProt Synonym Gene Names: C6ST-1; GST-0

Uniprot Description

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues of keratan sulfate, another glycosaminoglycan, and the Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. May play a role in the maintenance of naive T-lymphocytes in the spleen.