PTP-1B (Protein Tyrosine Phosphatase1B) Active Protein | PTP-1B active protein

PTP-1B (Protein Tyrosine Phosphatase1B), 1-321aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA203040

• Gene Names: PTPN1; PTP1B
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PTP-1B (Protein Tyrosine Phosphatase1B); 1-321aa; Human; E Coli (Bioactivity Validated); Protein tyrosine phosphatase non-receptor type 1; PTPN1; PTP1B; PTP-1B; Protein Tyrosine Phosphatase 1B; EC 3.1.3.48; Protein-tyrosine phosphatase1B; Tyrosine-protein phosphatase non-receptor type 1; Protein Tyrosine Phosphatase Non Receptor Type-1; PTP-1B active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 25 mM Tris-HCl, pH7.5, 2 mM beta-mercaptoethanol, 1 mM EDTA.1mMDTT, 20%glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-321aa
• Sequence: MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N

• Applicable Applications for PTP-1B active protein: Enzyme Activity, SDS-PAGE
• Tag: Non-Tagged
• Biological Activity: Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week.; For long term storage, aliquot and store at -20 degree C or -80 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomasie blue stain)

Related Product Information for PTP-1B active protein

The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.

Product Categories/Family for PTP-1B active protein

Signal Transduction

References

Chernoff J., et al. (1990) Proc. Natl. Acad. Sci. uSA. 87, 2735-9

NCBI and Uniprot Product Information

• NCBI GI #: 4506289
• NCBI GeneID: 5770
• NCBI Accession #: NP_002818
• NCBI GenBank Nucleotide #: NM_002827.3
• UniProt Accession #: P18031; Q5TGD8; Q9BQV9; Q9NQQ4
• Molecular Weight: 37.3 kDa (321 aa)
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: PTPN1
• NCBI Official Synonym Symbols: PTP1B
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• UniProt Gene Name: PTPN1
• UniProt Synonym Gene Names: PTP1B; PTP-1B

NCBI Description

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.