ACE-2 recombinant protein

Recombinant rat ACE-2 protein

Cat. Num. AAA201361

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: ACE-2; Recombinant rat ACE-2 protein; ACE2; angiotensin-converting enzyme2; Ace2; ACE-related carboxypeptidase; ACE-2 recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-740aa
• Sequence: QSLIEEKAES FLNKFNQEAE DLSYQSSLAS WNYNTNITEE NAQKMNEAAA KWSAFYEEQS KIAQNFSLQE IQNATIKRQL KALQQSGSSA LSPDKNKQLN TILNTMSTIY STGKVCNSMN PQECFLLEPG LDEIMATSTD YNRRLWAWEG WRAEVGKQLR PLYEEYVVLK NEMARANNYE DYGDYWRGDY EAEGVEGYNY NRNQLIEDVE NTFKEIKPLY EQLHAYVRTK LMEVYPSYIS PTGCLPAHLL GDMWGRFWTN LYPLTTPFLQ KPNIDVTDAM VNQSWDAERI FKEAEKFFVS VGLPQMTPGF WTNSMLTEPG DDRKVVCHPT AWDLGHGDFR IKMCTKVTMD NFLTAHHEMG HIQYDMAYAK QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLPSN FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFQDK IPREQWTKKW WEMKREIVGV VEPLPHDETY CDPASLFHVS NDYSFIRYYT RTIYQFQFQE ALCQAAKHDG PLHKCDISNS TEAGQKLLNM LSLGNSGPWT LALENVVGSR NMDVKPLLNY FQPLFVWLKE QNRNSTVGWS TDWSPYADQS IKVRISLKSA LGKNAYEWTD NEMYLFRSSV AYAMREYFSR EKNQTVPFGE ADVWVSDLKP RVSFNFFVTS PKNVSDIIPR SEVEEAIRMS RGRINDIFGL NDNSLEFLGI YPTLKPPYEP PVT

• Applicable Applications for ACE-2 recombinant protein: SDS-PAGE, Enzyme Activity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Rat
• Tag: His-Tag
• Predicted N-term: Gln18
• Bioactivity: Specific activity is > 150pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH per minute at pH7.5, at 25 degree C.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for ACE-2 recombinant protein

ACE-2, also known as angiotensin-converting enzyme 2, is carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. It is able to hydrolyze apelin-13 and dynorphin-13 with high efficiency and may be an important regulator of heart function. Recombinant rat ACE-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ACE-2 recombinant protein

Enzymes & Proteases; Virus; Proteins

References

Li W., et al. (2004) J. Virol. 78:11429-11433. Crackower M., A. et al. (2002) Nature 417:822-828.

NCBI and Uniprot Product Information

• NCBI GI #: 58865588
• NCBI GeneID: 302668
• NCBI Accession #: NP_001012006.1
• NCBI GenBank Nucleotide #: NM_001012006.1
• UniProt Accession #: Q5EGZ1
• Molecular Weight: 92,491 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: Ace2
• NCBI Protein Information: angiotensin-converting enzyme 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• UniProt Gene Name: Ace2

NCBI Description

enzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels [RGD, Feb 2006]

Uniprot Description

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.