Human Chemokine C C Motif Receptor 7 ELISA Kit | CCR7 elisa kit

Human Chemokine C C Motif Receptor 7 ELISA Kit

Cat. Num. AAA755253

• Gene Names: CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• Reactivity: Human
• Synonyms: Chemokine C C Motif Receptor 7; Human Chemokine C C Motif Receptor 7 ELISA Kit; CCR7 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250- 5000pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR7 elisa kit

Intended Uses: This CCL-7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCL-7. This ELISA kit for research use only!

Principle of the Assay: CCL-7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-7 antibody and an CCL-7-HRP conjugate. The assay sample and buffer are incubated together with CCL-7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-7 concentration since CCL-7 from samples and CCL-7-HRP conjugate compete for the anti-CCL-7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-7 from the sample, fewer sites are left to bind CCL-7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CCR7 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 119581073
• NCBI GeneID: 1236
• Molecular Weight: 42,874 Da
• NCBI Official Full Name: chemokine (C-C motif) receptor 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 7
• NCBI Official Symbol: CCR7
• NCBI Official Synonym Symbols: BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• NCBI Protein Information: C-C chemokine receptor type 7; MIP-3 beta receptor; CC chemokine receptor 7; Bukitt's lymphoma receptor 2; Epstein-Barr virus induced gene 1; EBV-induced G protein-coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; Epstein-Barr virus-induced G-protein coupled receptor 1
• UniProt Protein Name: C-C chemokine receptor type 7
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR7
• UniProt Synonym Gene Names: CMKBR7; EBI1; EVI1; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1
• UniProt Entry Name: CCR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q12-q21.2

Cellular Component: cell surface; integral to membrane; plasma membrane; intracellular; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity

Biological Process: positive regulation of cell adhesion; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; positive regulation of JNK cascade; response to lipopolysaccharide; elevation of cytosolic calcium ion concentration; positive regulation of T cell receptor signaling pathway; positive regulation of humoral immune response; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of cell-matrix adhesion; inflammatory response; positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of hypersensitivity; positive regulation of phosphoinositide 3-kinase activity; negative thymic T cell selection; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; regulation of interferon-gamma production; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; ruffle organization and biogenesis; regulation of interleukin-1 beta secretion; immune response