Prostatic Acid Phosphatase/ACPP Recombinant Protein | ACPP recombinant protein

Recombinant mouse Prostatic Acid Phosphatase/ACPP protein

Cat. Num. AAA201266

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Prostatic Acid Phosphatase/ACPP; Recombinant mouse Prostatic Acid Phosphatase/ACPP protein; acid phosphatase; prostate; ACP3; ACP-3; ACPP; EC 3.1.3.2; PAP; Prostatic Acid Phosphatase; prostatic acid phosphatase; 5-nucleotidase; 5'-NT; Acid phosphatase 3; Ecto-5'-nucleotidase; Fluoride-resistant acid phosphatase; FRAP; Thiamine monophosphatase; TMPase; A030005E02Rik; Lap; Ppal; ACPP recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 32-381aa
• Sequence: KELKFVTLVF RHGDRGPIET FPTDPITESS WPQGFGQLTQ WGMEQHYELG SYIRKRYGRF LNDTYKHDQI YIRSTDVDRT LMSAMTNLAA LFPPEGISIW NPRLLWQPIP VHTVSLSEDR LLYLPFRDCP RFEELKSETL ESEEFLKRLH PYKSFLDTLS SLSGFDDQDL FGIWSKVYDP LFCESVHNFT LPSWATEDAM IKLKELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILKNMK LATQPQKYKK LVMYSAHDTT VSGLQMALDV YNGVLPPYAS CHMMELYHDK GGHFVEMYYR NETQNEPYPL TLPGCTHSCP LEKFAELLDP VISQDWATEC MATSSHQGRN

• Applicable Applications for ACPP recombinant protein: SDS-PAGE, Enzyme Activity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Mouse
• Tag: His-Tag
• Predicted N-term: Lys32
• Bioactivity: Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH5.0 at 37C.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for ACPP recombinant protein

ACPP, also known as Prostatic Acid Phosphatase, is a member of histidine acid phosphatase family. Prostatic Acid Phosphatase/ACPP catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins. The activity optimum of Prostatic Acid Phosphatase/ACPP is in the pH range of 4 - 6, and the activity is inhibited by L(+)-tartrate. It has also lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Recombinant mouse Prostatic Acid Phosphatase/ACPP protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ACPP recombinant protein

Enzymes & Proteases; Cell Cycle; Proteins

References

Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351-357. Veeramani, S. et al.(2005) Endocr. Relat. Cancer 12:805-822.

NCBI and Uniprot Product Information

• UniProt Accession #: Q8CE08
• Molecular Weight: 41.3kDa (356aa)
• UniProt Protein Name: Prostatic acid phosphatase
• Protein Family: Prostatic acid phosphatase
• UniProt Gene Name: Acpp
• UniProt Synonym Gene Names: 5'-NT; FRAP; TMPase
• UniProt Entry Name: PPAP_MOUSE

Uniprot Description

ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.2; EC 3.1.3.5

Cellular Component: apical part of cell; extracellular region; extracellular space; filopodium; Golgi cisterna; integral to membrane; intracellular; lysosomal membrane; lysosome; membrane; multivesicular body; nucleus; plasma membrane; secretory granule; vesicle membrane

Molecular Function: 5'-nucleotidase activity; acid phosphatase activity; choline binding; hydrolase activity; identical protein binding; phosphoric monoester hydrolase activity; protein homodimerization activity; thiamin phosphate phosphatase activity

Biological Process: adenosine metabolic process; dephosphorylation; nucleotide metabolic process; positive regulation of adenosine receptor signaling pathway; purine base metabolic process; regulation of sensory perception of pain; thiamin metabolic process