Immunofluorescence (IF)
(Immunofluorescence staining of mouse SELL in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse SELL monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red).)
Rabbit anti-Mouse SELL/CD62L Monoclonal Antibody | anti-SELL antibody
Recombinant Anti-SELL/CD62L Antibody, Rabbit Monoclonal
Gene Names
Sell; Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
SELL/CD62L; Monoclonal Antibody; Recombinant Anti-SELL/CD62L Antibody; Rabbit Monoclonal; CD62L/L-Selectin Antibody; Rabbit MAb; selectin L; Anti-AI528707 Antibody; Anti-CD62L Antibody; Anti-L-selectin Antibody; Anti-LECAM-1 Antibody; Anti-Lnhr Antibody; Anti-Ly-22 Antibody; Anti-Ly-m22 Antibody; Anti-Lyam-1 Antibody; Anti-Lyam1 Antibody; anti-SELL antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
414
Specificity
Mouse CD62L/L-Selectin
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SELL antibody
Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD62L/L-Selectin Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD62L/L-Selectin (rM CD62L/L-Selectin).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of mouse SELL in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse SELL monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red).)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse SELL in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse SELL monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD62L expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD62L, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse CD62L expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD62L, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-SELL antibody
L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naive and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.
References
Smalley DM, et al. (2005) L-selectin: mechanisms and physiological significance of ectodomain cleavage. J Cell Mol Med. 9(2): 255-66.Grailer JJ, et al. (2009) L-selectin: role in regulating homeostasis and cutaneous inflammation. J Dermatol Sci. 56(3): 141-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,288 Da
NCBI Official Full Name
L-selectin isoform 1
NCBI Official Synonym Full Names
selectin, lymphocyte
NCBI Official Symbol
Sell
NCBI Official Synonym Symbols
Lnhr; CD62L; Ly-22; Lyam1; Ly-m22; Lyam-1; LECAM-1; AI528707; L-selectin
NCBI Protein Information
L-selectin; LAM-1; LECAM1; lymphocyte antigen 22; lymph node homing receptor; leukocyte adhesion molecule 1; CD62 antigen-like family member L; lymphocyte surface MEL-14 antigen; leukocyte-endothelial cell adhesion molecule 1
UniProt Protein Name
L-selectin
Protein Family
UniProt Gene Name
Sell
UniProt Synonym Gene Names
Lnhr; Ly-22; Ly22; LAM-1; LECAM1; Ly-22
UniProt Entry Name
LYAM1_MOUSE
Uniprot Description
SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; protease binding; cell adhesion molecule binding; carbohydrate binding; glycolipid binding
Biological Process: regulation of apoptosis; cell adhesion; response to ATP
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Product Notes
The SELL sell (Catalog #AAA8110849) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-SELL/CD62L Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SELL/CD62L can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the SELL sell for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SELL/CD62L, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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