Thymidine Phosphorylase Recombinant Protein | TP recombinant protein

Recombinant Thymidine Phosphorylase (TP)

Cat. Num. AAA2029940

• Gene Names: TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Thymidine Phosphorylase; Recombinant Thymidine Phosphorylase (TP); TP recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.4 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KR DGGHLREADI RNFVHAVIDG RAQDTQIGAM LMAIRLQGMN LEETSVLTRA LAESGQQLEW PKAWHQQLVD KHSTGGVGDK VSLVLAPALA ACGCKVPMIS GRSLGHTGGT LDKLESIPGF GVTQSPEQML HILEEVGCCI VGQSAKLVPA DGILYAARDV TATVDSVPLI TASILSKKAV EGLSTLVVDV KFGGAAVFPD QEKARELAKM LVRVGVSLGL KVAAALTAMD NPLGRSVGHT LEVEEALLCLDGAGPPDLRD LVIRLGGAIL WISGQ
• Sequence Length: 482

• Applicable Applications for TP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.9kDa
• Accurate Molecular Mass (KD): 32kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Lys29~Gln305
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 384229049
• NCBI GeneID: 1890
• NCBI Accession #: NP_001244917.1
• NCBI GenBank Nucleotide #: NM_001257988.1
• Molecular Weight: 50,405 Da
• NCBI Official Full Name: thymidine phosphorylase isoform 1 proprotein
• NCBI Official Synonym Full Names: thymidine phosphorylase
• NCBI Official Symbol: TYMP
• NCBI Official Synonym Symbols: TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
• NCBI Protein Information: thymidine phosphorylase
• UniProt Protein Name: Thymidine phosphorylase
• Protein Family: Thymidine phosphorylase
• UniProt Gene Name: TYMP
• UniProt Synonym Gene Names: ECGF1; TP; PD-ECGF
• UniProt Entry Name: TYPH_HUMAN

NCBI Description

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

Uniprot Description

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Cytokine; DNA replication; Phosphorylase; Motility/polarity/chemotaxis; EC 2.4.2.4; Transferase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; cell differentiation; DNA replication; chemotaxis

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)