Rabbit anti-Mouse RPL22 Polyclonal Antibody | anti-RPL22 antibody

RPL22, CT (RPL22, 60S ribosomal protein L22, EBER-associated protein, Epstein-Barr virus small RNA-associated protein, Heparin-binding protein HBp15) (PE)

Cat. Num. AAA6343423

• Gene Names: RPL22; EAP; L22; HBP15; HBP15/L22
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL22; Polyclonal Antibody; CT (RPL22; 60S ribosomal protein L22; EBER-associated protein; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15) (PE); Heparin-binding protein HBp15; anti-RPL22 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RPL22 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPL22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the C-terminal region of human RPL22.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPL22 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RPL22 antibody detected the RPL22 protein (arrow).)

Related Product Information for anti-RPL22 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.

Product Categories/Family for anti-RPL22 antibody

Antibodies; Binding Proteins

References

Houmani, J.L., et al. J. Virol. 83(19):9844-9853(2009). Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008). Fok, V., et al. RNA 12(5):872-882(2006). Nakao, K., et al. Otolaryngol Head Neck Surg 134(4):639-645(2006). Chen, K.C., et al. Urol. Int. 74(3):280-282(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4506613
• NCBI GeneID: 6146
• NCBI Accession #: NP_000974.1
• NCBI GenBank Nucleotide #: NM_000983.3
• UniProt Accession #: P35268; Q6IBD1; B2R495
• Molecular Weight: 14,787 Da
• NCBI Official Full Name: 60S ribosomal protein L22 proprotein
• NCBI Official Synonym Full Names: ribosomal protein L22
• NCBI Official Symbol: RPL22
• NCBI Official Synonym Symbols: EAP; L22; HBP15; HBP15/L22
• NCBI Protein Information: 60S ribosomal protein L22; EBER-associated protein; heparin-binding protein 15; heparin-binding protein HBp15; Epstein-Barr-encoded RNA-associated protein; Epstein-Barr virus small RNA-associated protein
• UniProt Protein Name: 60S ribosomal protein L22
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL22
• UniProt Synonym Gene Names: EAP
• UniProt Entry Name: RL22_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL22: a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p36.31

Cellular Component: focal adhesion; cytoplasm; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: heparin binding; protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; alpha-beta T cell differentiation; gene expression; viral transcription; viral infectious cycle; translational termination