Typical Standard Curve/Testing Data
Soluble Collagen Assay Kit
Soluble Collagen Assay Kit
Synonyms
Soluble Collagen; Soluble Collagen Assay Kit; Soluble Collagen assay kit
Preparation and Storage
Upon receipt, store the Sirius Red Reagent at room temperature and store the rest of the kit at 4 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Soluble Collagen assay kit
Background/Introduction: Collagen serves as the major structural component of animal connective tissues. Collagen is found in fibrous tissues such as skin and ligaments, but is also found in large amounts in bone, cartilage, and cornea. When hydrolyzed, collagen forms gelatin which is used in the food industry as well as medically to treat bone and skin disorders. Collagen makes up about 30% of the total protein content in animals, making it the most abundant animal protein. Hydroxyproline, a modified form of proline, is found almost exclusively in the protein collagen, in the Y position of the repeating tripeptide Gly-X-Y. By allowing sharp twisting of the collagen helix, hydroxyproline helps to stabilize the structure of collagen. Collagen plays an important structural role in the heart. The cardiac extracellular matrix, which is made up mostly of fibrillar collagen, preserves myocardial integrity, allows for heart force transmission, and contributes to myocyte orientation. Any interruption of the finely balanced regulation of collagen synthesis, post-synthetic assembly, post-translational modification and degradation may have large effects on myocardial function. In addition, collagen has several clinical uses. Collagen has been used extensively in cosmetic surgery to aid in the healing process for burn patients. Collagen has also been used to reconstruct bone in dental, orthopedic, and other surgical scenarios. Collagen is used for bone grafts because of its triple helical structure which makes it a very strong molecule. It is also optimal for use in bones because it does not compromise the structural integrity of the skeleton. Finally, both human and bovine collagen has been used as dermal fillers to treat wrinkling and skin aging. Soluble Collagen Assay Kit provides a convenient colorimetric method for the detection of soluble collagen from cell or tissue samples. First, the unknown samples or collagen standards are added to a 96 well plate and dried down overnight. Then, a Sirius Red reagent is added to stain the [Gly-x-y] triple helix structure of collagen. Finally, the stained collagen is washed with an Acidic Reagent, eluted from the plate with a Basic Reagent, transferred to a new 96 well plate and measured by a plate spectrophotometer. The amount of collagen in the unknown samples is determined by comparing with a predetermined collagen standard curve. The provided reagents are sufficient for the evaluation of 96 assays including standards and unknown samples.
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Product Notes
The Soluble Collagen (Catalog #AAA169274) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Soluble Collagen, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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