Typical Testing Data/Standard Curve (for reference only)
Formate Assay Kit
Formate Assay Kit (Colorimetric)
Synonyms
Formate; Formate Assay Kit (Colorimetric); Formate assay kit
Preparation and Storage
Upon receipt, store the 5X Assay Buffer at room temperature. Store the 50X NAD+ and Formate Dehydrogenase at -80 degree C. Store all remaining components at -20 degree C. The 10X Colorimetric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Formate assay kit
Principle of the Assay: The Formate Assay Kit is a sensitive, quantitative colorimetric assay for formate in a variety of sample types. The provided reagents are sufficient for the evaluation of 100 assays*. The unknown samples or formate standards are added to a 96 well plate followed by the Colorimetric Probe Mix containing WST-1, an electron mediator, and Formate Dehydrogenase (FDH). During a brief incubation the WST-1 is converted to the formazan form (Figure 1) and the absorbance of the plate is read at 450 nm. The content of formate in the unknown samples is determined by comparison with a predetermined formate standard curve. The kit has a detection sensitivity limit of 12.5 uM formate.
Background/Introduction: Formic acid is the simplest carboxylic acid with chemical formula CH2O2. It is often used as an antibacterial agent/preservative in livestock feed. In some countries it is applied to fresh hay in order to promote lactic acid fermentation and to lower butyric acid formation. Formic acid can block certain decay processes in feed which results in the feed retaining its nutritive value longer, and is therefore often employed to preserve cattle winter feed. It is sometimes added to poultry feed to kill E. coli bacteria. Formic acid is also used to tan leather, dye textiles, remove limescale, and clean toilet bowls. Beekeepers use formic acid to kill mites that can destroy bee colonies. In humans at low physiological concentrations, formic acid is metabolized easily and removed by the body (normal urine levels are about 280 uM). At higher doses, however, it can be toxic; in methanol poisoning it is the formic acid and formaldehyde metabolites rather than methanol that cause optic nerve damage resulting in blindness. Long term formic acid exposure in humans may cause kidney damage as well as the development of a skin allergy that appears when re-exposure to the chemical.
Background/Introduction: Formic acid is the simplest carboxylic acid with chemical formula CH2O2. It is often used as an antibacterial agent/preservative in livestock feed. In some countries it is applied to fresh hay in order to promote lactic acid fermentation and to lower butyric acid formation. Formic acid can block certain decay processes in feed which results in the feed retaining its nutritive value longer, and is therefore often employed to preserve cattle winter feed. It is sometimes added to poultry feed to kill E. coli bacteria. Formic acid is also used to tan leather, dye textiles, remove limescale, and clean toilet bowls. Beekeepers use formic acid to kill mites that can destroy bee colonies. In humans at low physiological concentrations, formic acid is metabolized easily and removed by the body (normal urine levels are about 280 uM). At higher doses, however, it can be toxic; in methanol poisoning it is the formic acid and formaldehyde metabolites rather than methanol that cause optic nerve damage resulting in blindness. Long term formic acid exposure in humans may cause kidney damage as well as the development of a skin allergy that appears when re-exposure to the chemical.
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Product Notes
The Formate (Catalog #AAA169590) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Formate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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