Typical Standard Curve/Testing Data
Pyruvate Assay Kit | AM587_10001230 assay kit
Pyruvate Assay Kit
Synonyms
Pyruvate; Pyruvate Assay Kit; AM587_10001230 assay kit
Sequence Length
597
Preparation and Storage
Upon receipt, store the Pyruvate Standard and 10X Assay Buffer at 4 degree C. Store all other components at -20 degree C. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for AM587_10001230 assay kit
Principle of the Assay: Pyruvate Assay Kit measures total pyruvate within biological samples. Pyruvate is oxidized by pyruvate oxidase in the presence of phosphate and oxygen into acetyl phosphate, carbon dioxide, and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of pyruvate standard within the 96-well microtiter plate format. Samples and standards are incubated for 30 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background/Introduction: Pyruvate, the conjugate base of pyruvic acid, is an alpha-keto acid that serves as a key intermediate in several metabolic pathways. Pyruvate can be synthesized from glucose through the glycolytic pathway and converted back to carbohydrates through gluconeogenesis. Pyruvate can be converted to fatty acids through a reaction involving acetyl-CoA and can also be used in the synthesis of the amino acid alanine. Pyruvate can be converted into ethanol or lactic acid through the process of fermentation. Finally, pyruvate is a key component in providing energy to cells through the citric acid cycle under oxygen rich conditions (aerobic respiration), and can also lead to production of lactate in anaerobic environments (fermentation). Pyruvate Assay Kit is a simple fluorometric assay that measures the amount of total pyruvate present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, pyruvate standards, and unknown samples. Sample pyruvate concentrations are determined by comparison with a known pyruvate standard. The kit has a detection sensitivity limit of 1 uM pyruvate.
Background/Introduction: Pyruvate, the conjugate base of pyruvic acid, is an alpha-keto acid that serves as a key intermediate in several metabolic pathways. Pyruvate can be synthesized from glucose through the glycolytic pathway and converted back to carbohydrates through gluconeogenesis. Pyruvate can be converted to fatty acids through a reaction involving acetyl-CoA and can also be used in the synthesis of the amino acid alanine. Pyruvate can be converted into ethanol or lactic acid through the process of fermentation. Finally, pyruvate is a key component in providing energy to cells through the citric acid cycle under oxygen rich conditions (aerobic respiration), and can also lead to production of lactate in anaerobic environments (fermentation). Pyruvate Assay Kit is a simple fluorometric assay that measures the amount of total pyruvate present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, pyruvate standards, and unknown samples. Sample pyruvate concentrations are determined by comparison with a known pyruvate standard. The kit has a detection sensitivity limit of 1 uM pyruvate.
Product Categories/Family for AM587_10001230 assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
65,102 Da
NCBI Official Full Name
Pyruvate
UniProt Protein Name
Pyruvate
Protein Family
UniProt Gene Name
AM587_10001230
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Product Notes
The Pyruvate am587_10001230 (Catalog #AAA169285) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Pyruvate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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