Typical Standard Curve/Testing Data
Branched Chain Amino Acid Assay Kit
Branched Chain Amino Acid Assay Kit
Synonyms
Branched Chain Amino Acid; Branched Chain Amino Acid Assay Kit; Branched Chain Amino Acid assay kit
Sequence Length
298
Preparation and Storage
Upon receipt, store the L-Leucine Standard, WST-1 Reagent, and Leucine Dehydrogenase at -20 degree C. The WST-1 reagent is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles. Store the NAD+ at -80 degree C. Store the 5X Assay Buffer at 4 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Branched Chain Amino Acid assay kit
Principle of the Assay: Branched Chain Amino Acid Assay Kit measures BCCAs within food or biological samples. L-Leucine, L-valine, and L-isoleucine are converted by Leucine Dehydrogenase (in the presence of excess NAD+) into their corresponding alpha-keto products (alpha-ketoisocaproate, alpha-ketovalerate, or alpha-ketoisovalerate) plus ammonia and NADH. The converted NADH is then detected colorimetrically with WST-1 which is converted to the formazan form in the presence of an electron mediator. Samples are compared to a known concentration of L-Leucine standard within the 96-well microtiter plate format. Samples and standards are then read with a standard 96-well colorimetric plate reader (Figure 1).
Background/Introduction: Amino acids are organic compounds that contain amine (-NH2) and carboxyl (-COOH) functional groups, as well as a side-chain (R group) which confers uniqueness to each amino acid. The main elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, although other elements can be found in some amino acids. About 500 amino acids are known, but only 20 are coded in the human genome. A Branched Chain Amino Acid (BCAA) contains a branch structure side chain with one central carbon connected to at least three other carbon atoms. The only three BCAAs coded by the human genome are leucine, valine, and isoleucine. BCAAs play key roles in animal metabolism and physiology. BCAAs enhance protein synthesis, signaling pathways, and glucose metabolism. On a physiological level, BCAAs function within the immune system. BCAAs are degraded by dehydrogenase and decarboxylase enzymes, are found in immune cells, and are required for lymphocyte growth, proliferation, and activity. BCAAs are transported into the brain by the same enzyme used to transport aromatic amino acids (tryptophan, tyrosine, and phenylalanine). Once in the brain BCAAs contribute to protein synthesis, neurotransmitter synthesis, and energy production. In humans, rats and mice models, BCAA levels in the blood are elevated in obese, insulin resistant models of diet-induced diabetes. This result suggests the possibility that BCAAs promote obesity and diabetes pathogenesis. In mice fed limited amounts of BCAAs, glucose tolerance is improved and weight loss is observed. Branched Chain Amino Acid Assay Kit is a simple colorimetric assay that measures the total amount of free BCAAs (Leucine, Isoleucine, and Valine) present in foods or biological samples in a 96-well microtiter plate format. BCAAs in polypeptide chains (peptides and proteins) are not detected. Each kit provides sufficient reagents to perform up to 192 assays*, including blanks, L-Leucine standards and unknown samples. Sample BCAA concentrations are determined by comparison with a known L-Leucine standard. The kit has a detection sensitivity limit of 15.6 uM BCAAs.
Background/Introduction: Amino acids are organic compounds that contain amine (-NH2) and carboxyl (-COOH) functional groups, as well as a side-chain (R group) which confers uniqueness to each amino acid. The main elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, although other elements can be found in some amino acids. About 500 amino acids are known, but only 20 are coded in the human genome. A Branched Chain Amino Acid (BCAA) contains a branch structure side chain with one central carbon connected to at least three other carbon atoms. The only three BCAAs coded by the human genome are leucine, valine, and isoleucine. BCAAs play key roles in animal metabolism and physiology. BCAAs enhance protein synthesis, signaling pathways, and glucose metabolism. On a physiological level, BCAAs function within the immune system. BCAAs are degraded by dehydrogenase and decarboxylase enzymes, are found in immune cells, and are required for lymphocyte growth, proliferation, and activity. BCAAs are transported into the brain by the same enzyme used to transport aromatic amino acids (tryptophan, tyrosine, and phenylalanine). Once in the brain BCAAs contribute to protein synthesis, neurotransmitter synthesis, and energy production. In humans, rats and mice models, BCAA levels in the blood are elevated in obese, insulin resistant models of diet-induced diabetes. This result suggests the possibility that BCAAs promote obesity and diabetes pathogenesis. In mice fed limited amounts of BCAAs, glucose tolerance is improved and weight loss is observed. Branched Chain Amino Acid Assay Kit is a simple colorimetric assay that measures the total amount of free BCAAs (Leucine, Isoleucine, and Valine) present in foods or biological samples in a 96-well microtiter plate format. BCAAs in polypeptide chains (peptides and proteins) are not detected. Each kit provides sufficient reagents to perform up to 192 assays*, including blanks, L-Leucine standards and unknown samples. Sample BCAA concentrations are determined by comparison with a known L-Leucine standard. The kit has a detection sensitivity limit of 15.6 uM BCAAs.
Product Categories/Family for Branched Chain Amino Acid assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Branched chain amino acid
Similar Products
Product Notes
The Branched Chain Amino Acid (Catalog #AAA169297) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Branched Chain Amino Acid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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