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Typical Testing Data/Standard Curve (for reference only)

Gentamicin ELISA Kit

Gentamicin Competitive ELISA Kit

Synonyms
Gentamicin; Gentamicin Competitive ELISA Kit; Gentamicin elisa kit
Ordering
For Research Use Only!
Preparation and Storage
Upon receipt, store Anti-Gentamicin Antibody (500X), Secondary Antibody, HRP Conjugate (1000X), Gentamicin Standard, and Gentamicin Conjugate at -20 degree C. Store all the remaining components at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Gentamicin elisa kit
Principle of the Assay: First, a gentamicin conjugate is coated on an ELISA plate. The unknown gentamicin samples or gentamicin standards are then added to the gentamicin conjugate preabsorbed ELISA plate. After a brief incubation, an anti-gentamicin monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The total content of gentamicin in unknown extracted samples is determined by comparison with a gentamicin standard curve.

Background/Introduction: Gentamicin is an antibiotic that has been used to treat various types of bacterial infections such as pelvic inflammatory disease, pneumonia, bone infections, endocarditis, meningitis, sepis, and urinary tract infections. Gentamicin was patented in 1962 and approved to be used medically in 1964. Gentamicin can be administered topically, intravenously, or by injection into a muscle. Gentamicin is an aminoglycoside that works by blocking synthesis of bacterial proteins resulting in bacterial death. This antibiotic is made from the bacterium Micromonospora purpurea. As a side effect of treatment, gentamicin can cause inner ear problems (such as hearing loss or poor balance) as well as kidney problems. In addition, gentamicin can cause harm to a developing baby in pregnant women. However, the antibiotic appears to be safe when administered to women who are breastfeeding newborns. Gentamicin is also used by researchers as an antibacterial agent in cell cultures. Gentamicin is a very heat-stable antibiotic, remaining active even after autoclaving, which makes the antibiotic useful in the manufacturing of some types of microbiological growth media. ???????? Gentamicin Competitive ELISA Kit provides a convenient method for the detection of total Gentamicin in extracts from cells, tissue, serum, plasma, urine, or foods. The total content of gentamicin in unknown samples is determined by comparison with a gentamicin standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 25 nM gentamicin.
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Product Notes

The Gentamicin (Catalog #AAA169591) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Gentamicin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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