Typical Testing Data/Standard Curve (for reference only)
Glycine Assay Kit
Glycine Assay Kit
Synonyms
Glycine; Glycine Assay Kit; Glycine assay kit
Preparation and Storage
Upon receipt, store the 1X Assay Buffer at room temperature. Store all other components at -20 degree C. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Glycine assay kit
Principle of the Assay: Glycine Assay Kit measures glycine within food or biological samples. Glycine is deaminated by glycine oxidase into glyoxylate plus ammonia and hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are read with a standard 96-well fluorometric plate reader. Samples are compared to a known concentration of glycine standard within the 96-well microtiter plate format (Figure 1).
Background/Introduction: Glycine is an amino acid containing amine (-NH2) and carboxyl (-COOH) functional groups, as well as a single hydrogen as its side-chain. Glycine is a non essential amino acid since it is synthesized in the body from the amino acid serine. In most organisms, the enzyme serine hydroxymethyltransferase uses the cofactor pyridoxal phosphate to convert serine (in the presence of tetrahydrofolate) to glycine and methylene tetrahydrofolate. In the vertebrate liver, glycine synthase is used to convert methylene tetrahydrofolate and NADH to glycine, tetrahydrofolate, and NAD+. Glycine is one of 20 natural amino acids that serve as fundamental building blocks for the production of proteins. In conjunction with hydroxyproline, glycine makes up the helical structure of collagen. In higher eukaryotes, glycine and succinyl-CoA are used by aminolevulinic acid synthase to make delta- aminolevulinic acid, the main precursor to porphyrins. In the central nervous system, glycine serves as an inhibitory neurotransmitter in the spinal cord, acting as an NMDA receptor co-agonist with glutamate. Glycine supplements have been shown to improve sleep quality, and glycine used in conjunction with other common drugs has been proven beneficial in treating schizophrenia. Bile acids, which are steroid acids found predominantly in the bile of mammals and other vertebrates, are conjugated with glycine (as well as taurine) in the liver, forming primary bile acids. Glycine Assay Kit is a simple fluorometric assay that measures the amount of free glycine present in foods or biological samples in a 96-well microtiter plate format. Glycine found in polypeptide chains (peptides and proteins) or other conjugated molecules (primary bile acids) are not detected. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, glycine standards and unknown samples. Sample glycine concentrations are determined by comparison with a known glycine standard. The kit has a detection sensitivity limit of 1.56 uM glycine.
Background/Introduction: Glycine is an amino acid containing amine (-NH2) and carboxyl (-COOH) functional groups, as well as a single hydrogen as its side-chain. Glycine is a non essential amino acid since it is synthesized in the body from the amino acid serine. In most organisms, the enzyme serine hydroxymethyltransferase uses the cofactor pyridoxal phosphate to convert serine (in the presence of tetrahydrofolate) to glycine and methylene tetrahydrofolate. In the vertebrate liver, glycine synthase is used to convert methylene tetrahydrofolate and NADH to glycine, tetrahydrofolate, and NAD+. Glycine is one of 20 natural amino acids that serve as fundamental building blocks for the production of proteins. In conjunction with hydroxyproline, glycine makes up the helical structure of collagen. In higher eukaryotes, glycine and succinyl-CoA are used by aminolevulinic acid synthase to make delta- aminolevulinic acid, the main precursor to porphyrins. In the central nervous system, glycine serves as an inhibitory neurotransmitter in the spinal cord, acting as an NMDA receptor co-agonist with glutamate. Glycine supplements have been shown to improve sleep quality, and glycine used in conjunction with other common drugs has been proven beneficial in treating schizophrenia. Bile acids, which are steroid acids found predominantly in the bile of mammals and other vertebrates, are conjugated with glycine (as well as taurine) in the liver, forming primary bile acids. Glycine Assay Kit is a simple fluorometric assay that measures the amount of free glycine present in foods or biological samples in a 96-well microtiter plate format. Glycine found in polypeptide chains (peptides and proteins) or other conjugated molecules (primary bile acids) are not detected. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, glycine standards and unknown samples. Sample glycine concentrations are determined by comparison with a known glycine standard. The kit has a detection sensitivity limit of 1.56 uM glycine.
Product Categories/Family for Glycine assay kit
Similar Products
Product Notes
The Glycine (Catalog #AAA169316) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glycine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
