Typical Standard Curve/Testing Data
Starch Assay Kit
Starch Assay Kit (Fluorometric)
Synonyms
Starch; Starch Assay Kit (Fluorometric); Starch assay kit
Preparation and Storage
Upon receipt, store the 10X Assay Buffer at room temperature. Store all other components at -20 degree C. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Starch assay kit
Principle of the Assay: Starch Assay Kit measures total starch within biological samples. Starch is broken down into glucose monomers by amyloglucosidase first, glucose is then oxidized by glucose oxidase into D-gluconic acid and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples are compared to a known concentration of Starch standard within the 96-well microtiter plate format. Samples and standards are incubated for 45 minutes and then read with a standard 96-well fluorometric plate reader (Figure 1).
Background/Introduction: Starch is a polymeric carbohydrate made of a long chain of glucose units joined together by covalent glycosidic bonds. This polymer is created by most green plants as a form of reserve energy. Plants make starch by using the enzyme glucose-1-phosphate adenylyltransferase to convert glucose 1-phosphate to ADP-glucose (this initial step requires ATP as a source of energy). ADP-glucose is then added to the elongating chain of glucose residues by the enzyme starch synthase. This step releases ADP and creates amylose. Finally, a starch branching enzyme creates 1,6-alpha glycosidic bonds between these chains, creating the branched amylopectin molecule. Starch is the most common carbohydrate in the diet of humans and can be found in many common foods such as potatoes, corn, wheat, and rice. Foods such as potato have a higher glycemic index rating due to high amounts of digestible starch, thus increasing the risk of type-2 diabetes and cardiovascular disease in consumers. Food starches are often used as thickeners and stabilizers in foods such as soups, sauces, puddings, custards, gravies, pie fillings, and salad dressings, as well as to make various types of noodles. Starch is often converted into sugar which can be used in processed foods. Starch is used in the paper industry as an adhesive, and often is used to stiffen some parts of clothing just prior to ironing. Starch Assay Kit is a simple fluorometric assay that measures the amount of total starch present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, starch standards, and unknown samples. Sample starch concentrations are determined by comparison with a known starch standard. The kit has a detection sensitivity limit of 625 ng/mL starch.
Background/Introduction: Starch is a polymeric carbohydrate made of a long chain of glucose units joined together by covalent glycosidic bonds. This polymer is created by most green plants as a form of reserve energy. Plants make starch by using the enzyme glucose-1-phosphate adenylyltransferase to convert glucose 1-phosphate to ADP-glucose (this initial step requires ATP as a source of energy). ADP-glucose is then added to the elongating chain of glucose residues by the enzyme starch synthase. This step releases ADP and creates amylose. Finally, a starch branching enzyme creates 1,6-alpha glycosidic bonds between these chains, creating the branched amylopectin molecule. Starch is the most common carbohydrate in the diet of humans and can be found in many common foods such as potatoes, corn, wheat, and rice. Foods such as potato have a higher glycemic index rating due to high amounts of digestible starch, thus increasing the risk of type-2 diabetes and cardiovascular disease in consumers. Food starches are often used as thickeners and stabilizers in foods such as soups, sauces, puddings, custards, gravies, pie fillings, and salad dressings, as well as to make various types of noodles. Starch is often converted into sugar which can be used in processed foods. Starch is used in the paper industry as an adhesive, and often is used to stiffen some parts of clothing just prior to ironing. Starch Assay Kit is a simple fluorometric assay that measures the amount of total starch present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays*, including blanks, starch standards, and unknown samples. Sample starch concentrations are determined by comparison with a known starch standard. The kit has a detection sensitivity limit of 625 ng/mL starch.
Product Categories/Family for Starch assay kit
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Product Notes
The Starch (Catalog #AAA169282) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Starch, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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