Typical Testing Data/Standard Curve (for reference only)
alpha-Ketoglutarate Assay Kit
alpha-Ketoglutarate Assay Kit (Colorimetric)
Synonyms
alpha-Ketoglutarate; alpha-Ketoglutarate Assay Kit (Colorimetric); alpha-Ketoglutarate assay kit
Preparation and Storage
Upon receipt, store the alpha-KG Substrate Mix and 10X Assay Buffer at 4 degree C. Store the remaining components at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for alpha-Ketoglutarate assay kit
Principle of the Assay: The alpha-Ketoglutarate Assay Kit measures alpha-ketoglutarate through coupled enzymatic reactions. alpha-Ketoglutarate is transaminated with the production of pyruvate. Pyruvate is then detected with the colorimetric probe. Samples and standards are incubated for 60-120 minutes and then read with a standard 96-well specrophotometric plate reader (540-570 nm). Samples are compared to a known concentration of alpha-ketoglutarate standard within the 96-well microtiter plate format.
Background/Introduction: alpha-Ketoglutarate (alpha-KG, or 2-oxoglutarate), is an important molecule with a variety of roles within multiple metabolic pathways. alpha-KG is produced by the oxidative decarboxylation of isocitrate via isocitrate dehydrogenase and is also the keto acid product of the deamination of glutamate via glutamate dehydrogenase. alpha-Ketoglutarate exerts its influence in the tricarboxylic acid (TCA) cycle as a rate determining intermediary of cellular energy metabolism. Anaplerotic reactions can refuel the cycle by generating alpha-ketoglutarate from glutamate via glutamate dehydrogenase. alpha-Ketoglutarate acts as a nitrogen transporter that facilitates amino acid synthesis, while also inhibiting protein degradation in muscles. L-Alanine or L-Asparate can react with alpha-ketoglutarate via the transaminase enzymes alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to produce glutamate, which is the basis of nitrogen utilization and elimination. Amino groups from amino acids attach to alpha-KG where they are transported to the liver and can enter the urea cycle. These transamination and deamination pathways are also important for ammonia detoxification, as well as formation of the neurotrasmiters glutamate and gamma-aminobutyric acid (GABA). Other characteristics of alpha-ketoglutarate include antioxidant activity by quenching of hydrogen peroxide build up and facilitating its elimination. It also generates ATP via oxidative decarboxylation for energy in intestinal cell processes and redox homeostasis. The molecule can influence hydroxylation of proline to hydroxyproline, thus increasing the formation of collagen and bone matrix formation. Recently, alpha-KG has been noted to extend cell lifespan and delay age-related diseases (Figure 1).The alpha-Ketoglutarate Assay Kit is a simple colorimetric assay that measures the activity of alpha-ketoglutarate present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and samples. The kit has a detection sensitivity limit of ~3.9 uM alpha-ketoglutarate.
Background/Introduction: alpha-Ketoglutarate (alpha-KG, or 2-oxoglutarate), is an important molecule with a variety of roles within multiple metabolic pathways. alpha-KG is produced by the oxidative decarboxylation of isocitrate via isocitrate dehydrogenase and is also the keto acid product of the deamination of glutamate via glutamate dehydrogenase. alpha-Ketoglutarate exerts its influence in the tricarboxylic acid (TCA) cycle as a rate determining intermediary of cellular energy metabolism. Anaplerotic reactions can refuel the cycle by generating alpha-ketoglutarate from glutamate via glutamate dehydrogenase. alpha-Ketoglutarate acts as a nitrogen transporter that facilitates amino acid synthesis, while also inhibiting protein degradation in muscles. L-Alanine or L-Asparate can react with alpha-ketoglutarate via the transaminase enzymes alanine aminotransferase (ALT) or aspartate aminotransferase (AST) to produce glutamate, which is the basis of nitrogen utilization and elimination. Amino groups from amino acids attach to alpha-KG where they are transported to the liver and can enter the urea cycle. These transamination and deamination pathways are also important for ammonia detoxification, as well as formation of the neurotrasmiters glutamate and gamma-aminobutyric acid (GABA). Other characteristics of alpha-ketoglutarate include antioxidant activity by quenching of hydrogen peroxide build up and facilitating its elimination. It also generates ATP via oxidative decarboxylation for energy in intestinal cell processes and redox homeostasis. The molecule can influence hydroxylation of proline to hydroxyproline, thus increasing the formation of collagen and bone matrix formation. Recently, alpha-KG has been noted to extend cell lifespan and delay age-related diseases (Figure 1).The alpha-Ketoglutarate Assay Kit is a simple colorimetric assay that measures the activity of alpha-ketoglutarate present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and samples. The kit has a detection sensitivity limit of ~3.9 uM alpha-ketoglutarate.
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Product Notes
The alpha-Ketoglutarate (Catalog #AAA169588) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "alpha-Ketoglutarate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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