Typical Standard Curve/Testing Data
Lactate Assay Kit
Lactate Assay Kit (Fluorometric)
Synonyms
Lactate; Lactate Assay Kit (Fluorometric); Lactate assay kit
Preparation and Storage
Upon receipt, store the Lactate Standard, Fluorometric Probe, HRP, and Lactate Oxidase at -20 degree C. The Fluorescence Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles. Store the 10X Assay Buffer at room temperature.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Lactate assay kit
Principle of the Assay: Lactate Assay Kit measures lactate within biological samples. Lactate is oxidized by lactate oxidase into pyruvate and hydrogen peroxide. The hydrogen peroxide is then detected with a highly specific Fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and hydrogen peroxide, which bind in a 1:1 ratio. Samples and standards are incubated for 30-45 minutes and then read with a standard 96-well Fluorometric plate reader (Figure 1). Samples are compared to a known concentration of lactate standard within the 96-well microtiter plate format.
Background/Introduction: Lactic Acid is an alpha hydroxyl acid that can ionize a carboxyl proton to yield the lactate ion, the latter of which exists as two optical isomers L-Lactate and D-Lactate. The enzyme lactate dehydrogenase catalyzes the conversion of pyruvate to lactate in animals during the process of fermentation. Depending on the levels of exercise, blood levels of lactate can vary between 1 and 20 mM. In medicine, lactate is a component of intravenous fluids such as Hartmann's solution. These fluids are often used when blood loss occurs due to surgery or injury. In the brain, lactate, like glucose, is thought to be one of the main sources of energy. High levels of lactate have been found in the extracellular fluid surrounding neurons due to the high metabolic activity of glial cells. In the food industry, lactic acid is found in cheeses, milk, and various breads. In winemaking, lactic acid bacteria are used to reduce malic acid levels and therefore decrease the sharpness in flavor. Finally, in the detergent industry lactic acid has been used as an anti-bacterial agent as well as a soap-scum removal agent and descaler. Lactate Assay Kit (Fluorometric) is a simple fluorometric assay that measures the amount of total lactate present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, lactate standards and unknown samples. Sample lactate concentrations are determined by comparison with a known lactate standard. The kit has a detection sensitivity limit of 1.5 uM L-Lactate.
Background/Introduction: Lactic Acid is an alpha hydroxyl acid that can ionize a carboxyl proton to yield the lactate ion, the latter of which exists as two optical isomers L-Lactate and D-Lactate. The enzyme lactate dehydrogenase catalyzes the conversion of pyruvate to lactate in animals during the process of fermentation. Depending on the levels of exercise, blood levels of lactate can vary between 1 and 20 mM. In medicine, lactate is a component of intravenous fluids such as Hartmann's solution. These fluids are often used when blood loss occurs due to surgery or injury. In the brain, lactate, like glucose, is thought to be one of the main sources of energy. High levels of lactate have been found in the extracellular fluid surrounding neurons due to the high metabolic activity of glial cells. In the food industry, lactic acid is found in cheeses, milk, and various breads. In winemaking, lactic acid bacteria are used to reduce malic acid levels and therefore decrease the sharpness in flavor. Finally, in the detergent industry lactic acid has been used as an anti-bacterial agent as well as a soap-scum removal agent and descaler. Lactate Assay Kit (Fluorometric) is a simple fluorometric assay that measures the amount of total lactate present in biological samples in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, lactate standards and unknown samples. Sample lactate concentrations are determined by comparison with a known lactate standard. The kit has a detection sensitivity limit of 1.5 uM L-Lactate.
Product Categories/Family for Lactate assay kit
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Product Notes
The Lactate (Catalog #AAA169271) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Lactate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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