Typical Testing Data/Standard Curve (for reference only)
PLTP Activity II Assay Kit
PLTP Activity Fluorometric Assay Kit II
Synonyms
PLTP Activity II; PLTP Activity Fluorometric Assay Kit II; Phospholipid Transfer Protein Activity Fluorometric Assay Kit II; PLTP Activity II assay kit
Application Notes
Measurement of PLTP activity in animal serum, plasma and recombinant protein
Preparation and Storage
4 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLTP Activity II assay kit
Background/Introduction: Phosphodiesterase (PDE) is a family of enzymes that cleave a phosphodiester bond. These include cyclic nucleotide phosphodiesterases, phospholipase C and D, RNases, DNases and some restriction endonucleases. Phosphodiesterases are important enzymes used for a variety of biological functions including metabolism of second messenger molecules like cAMP / cGMP (therefore, regulation of their intracellular levels). PDE activity is responsible for degradation of mRNA after it had been translated to protein and of DNA during apoptosis. Some PDE isoforms such as PDE 5 and PDE 11 are therapeutic targets because of their role in the cardiovascular system and tumor formation respectively. BioVision's PDE assay kit is a fluorometric plate-based assay for kinetic measurement of total phosphodiesterase activity. It is based on the cleavage of a coumarin based synthetic substrate by phosphodiesterases to generate a product, which fluoresces at Ex/Em= 370/450 nm. It does not rely on the quantification of phosphate released by phosphodiesterase action, like other traditional methods currently in use. Therefore, sample background is negligible, and does not interfere with the assay. The substrate is specific for phosphodiesterases and is not cleaved by enzymes that act on carboxyl esters or by phosphatases. This assay can detect as low as 0.25 uU of total PDE.
Product Categories/Family for PLTP Activity II assay kit
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Product Notes
The PLTP Activity II (Catalog #AAA841605) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of PLTP activity in animal serum, plasma and recombinant protein. Researchers should empirically determine the suitability of the PLTP Activity II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLTP Activity II, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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