Typical Testing Data/Standard Curve (for reference only)
Acetoacetate Assay Kit
Acetoacetate Colorimetric Assay Kit
Synonyms
Acetoacetate; Acetoacetate Colorimetric Assay Kit; Acetoacetate assay kit
Application Notes
Measurement of acetoacetate in various biological samples.
Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.
Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Acetoacetate assay kit
Background/Introduction: Acetoacetate (AcAc), a beta-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-beta-hydroxybutyrate (beta-HB), or decarboxylated producing acetone (CH3)2CO). Ketone bodies (betaHB: 78%; AcAc: 20% & CH3)2CO: 2%) are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-beta-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 6M.
Product Categories/Family for Acetoacetate assay kit
Similar Products
Product Notes
The Acetoacetate (Catalog #AAA841853) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of acetoacetate in various biological samples. Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc. Researchers should empirically determine the suitability of the Acetoacetate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acetoacetate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
