Typical Testing Data/Standard Curve (for reference only)
Fumarate Assay Kit
Fumarate Colorimetric Assay Kit
Synonyms
Fumarate; Fumarate Colorimetric Assay Kit; Fumarate assay kit
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes ~ 40 minutes
Fast and convenient
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Fumarate assay kit
Product Information: Fumarate Assay Kit: Colorimetric Assay to detect fumarate in a variety of samples such as Serum, Cells, Tissues etc. within 40 min. Rapid, Convenient & Sensitive. Detection limit: 1 nmol. 100 Assays.
Background: Fumarate (HO?CCH=CHCO?H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. MyBioSource's Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (lambda = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 uM).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Application- Kit can detect as low as 1 nmol of fumarate per well (20 uM).
Background: Fumarate (HO?CCH=CHCO?H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. MyBioSource's Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (lambda = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 uM).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Application- Kit can detect as low as 1 nmol of fumarate per well (20 uM).
Product Categories/Family for Fumarate assay kit
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Product Notes
The Fumarate (Catalog #AAA841565) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fumarate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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