Typical Testing Data/Standard Curve (for reference only)
Isocitrate Assay Kit
Isocitrate Colorimetric Assay Kit
Synonyms
Isocitrate; Isocitrate Colorimetric Assay Kit; Isocitrate assay kit
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of isocitrate levels
Fast and convenient
Kit contains all necessary reagents for accurate measurement of isocitrate levels
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Isocitrate assay kit
Product Information: Isocitrate Assay Kit: Colorimetric Assay for detecting Isocitrate in variety of Samples such as Tissues, Cells, Food, Beverage Samples etc. within 40 min. Rapid, Convenient & Reliable. Detection Range: 1 to 20 nmoles (~0.2 - 5 mug). 100 Assays.
Background: Isocitric acid (HOOC-CHOH-CH (-COOH)-CH?-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and alpha-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to alpha-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. MyBioSource's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a lambdamax of 450nm. The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 - 5 ug) of isocitrate.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Application- Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 - 5 ug) of isocitrate.
Background: Isocitric acid (HOOC-CHOH-CH (-COOH)-CH?-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and alpha-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to alpha-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. MyBioSource's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a lambdamax of 450nm. The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 - 5 ug) of isocitrate.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Application- Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 - 5 ug) of isocitrate.
Product Categories/Family for Isocitrate assay kit
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Product Notes
The Isocitrate (Catalog #AAA841791) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Isocitrate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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