Typical Testing Data/Standard Curve (for reference only)
Nickel Assay Kit
Nickel Colorimetric Assay Kit
Synonyms
Nickel; Nickel Colorimetric Assay Kit; Nickel assay kit
Sequence Length
302
Samples
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes less than ~40 minutes
Fast and convenient
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Nickel assay kit
Product Information: Nickel Assay Kit: Simple Accurate Colorimetric Method of Quantitating Nickel in a variety of samples within 40 min. 100 Assays.
Background: Nickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO? to CH?) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. MyBioSource's Nickel Assay kit provides a simple method of quantifying Ni in a variety of samples. The assay takes advantage of reaction of Ni with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe and Co interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn, Cu, Zn) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
Summary: Detection method- Absorbance (300 to 600 nm)
Species reactivity- Mammalian
Applications- The assay is a simple method of quantifying Ni in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
Background: Nickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO? to CH?) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. MyBioSource's Nickel Assay kit provides a simple method of quantifying Ni in a variety of samples. The assay takes advantage of reaction of Ni with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe and Co interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn, Cu, Zn) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
Summary: Detection method- Absorbance (300 to 600 nm)
Species reactivity- Mammalian
Applications- The assay is a simple method of quantifying Ni in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
Product Categories/Family for Nickel assay kit
NCBI and Uniprot Product Information
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Product Notes
The Nickel (Catalog #AAA841803) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nickel, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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