Typical Testing Data/Standard Curve (for reference only)
Acid Phosphatase Assay Kit | PHOA assay kit
Acid Phosphatase Activity Colorimetric Assay Kit
Synonyms
Acid Phosphatase; Acid Phosphatase Activity Colorimetric Assay Kit; PHOA assay kit
Sequence Length
412
Samples
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
The assay detects acid phosphatase (AP) activity conveniently in various biological samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PHOA assay kit
Product Information: Acid Phosphatase Activity Colorimetric Assay Kit: High-Throughput Ready Colorimetric Assay to Measure Acid Phosphatase (AP) activity in Cells, Tissues, Serum, Plasma, Urine, Semen and Cell Culture Media. High sensitivity, Simple & Direct. 500 Assays.
Background: Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. MyBioSource's Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (lambdamax = 405 nm) when dephosphorylated by AP. The kit can detect uU acid phosphatase activity in samples.
Summary: Detection method- Absorbance (405 nm)
Species reactivity- Mammalian
Applications- Phosphate concentrations between 1 uM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Background: Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. MyBioSource's Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (lambdamax = 405 nm) when dephosphorylated by AP. The kit can detect uU acid phosphatase activity in samples.
Summary: Detection method- Absorbance (405 nm)
Species reactivity- Mammalian
Applications- Phosphate concentrations between 1 uM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Product Categories/Family for PHOA assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
45,628 Da
NCBI Official Full Name
acid phosphatase
UniProt Protein Name
Phosphate-repressible acid phosphatase
Protein Family
UniProt Gene Name
PHOA
UniProt Entry Name
PHOA_PENCH
Similar Products
Product Notes
The Acid Phosphatase phoa (Catalog #AAA841819) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Acid Phosphatase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
